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http://hdl.handle.net/2445/105206
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DC Field | Value | Language |
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dc.contributor.author | Ruíz Martínez, Marc | - |
dc.contributor.author | Navarro Ponz, Alfons | - |
dc.contributor.author | Marrades Sicart, Ramon Ma. | - |
dc.contributor.author | Viñolas Segarra, Núria | - |
dc.contributor.author | Santasusagna, Sandra | - |
dc.contributor.author | Muñoz García, Carmen | - |
dc.contributor.author | Ramírez Ruz, J. (José) | - |
dc.contributor.author | Molins López-Rodó, Laureano | - |
dc.contributor.author | Monzó Planella, Mariano | - |
dc.date.accessioned | 2017-01-02T16:55:36Z | - |
dc.date.available | 2017-01-02T16:55:36Z | - |
dc.date.issued | 2016-06-06 | - |
dc.identifier.issn | 1949-2553 | - |
dc.identifier.uri | http://hdl.handle.net/2445/105206 | - |
dc.description.abstract | BACKGROUND: Cancer-derived exosomes are involved in metastasis. YKT6 is a SNARE protein that participates in the regulation of exosome production and release, but its role in non-small cell lung cancer (NSCLC) has not been examined. MATERIALS AND METHODS: Ultracentrifugation-purified exosomes from the A549 cell line were studied by CRYO-TEM, nanoparticle tracking analysis and western blot (TSG101 marker). YKT6 was inhibited using a DsiRNA and selected pre-microRNAs. MicroRNAs targeting YKT6 were validated by Renilla/Luciferase assay and western blot. YKT6 expression and its prognostic impact were analyzed in 98 tissue specimens from resected NSCLC patients. RESULTS: Membranous nanosized vesicles (mode size: 128nm) with TSG101 protein were purified from A549 cells. YKT6 inhibition reduced exosome release by 80.9%. We validated miR-134 and miR-135b as miRNAs targeting YKT6, and transfection with the pre-miRNAs also produced a significant reduction in exosome release. The analysis of YKT6 in tumor samples showed that patients with high levels had shorter disease-free and overall survival. CONCLUSIONS: YKT6 is a key molecule in the regulation of exosome release in lung cancer cells and is in turn precisely regulated by miR-134 and miR-135b. Moreover, YKT6 levels impact prognosis of resected NSCLC patients. | - |
dc.format.extent | 10 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Impact Journals | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.18632/oncotarget.9862 | - |
dc.relation.ispartof | Oncotarget, 2016, vol. 7, num. 32, p. 51515-51524 | - |
dc.relation.uri | https://doi.org/10.18632/oncotarget.9862 | - |
dc.rights | cc-by (c) Ruíz Martínez, Marc et al., 2016 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Cirurgia i Especialitats Medicoquirúrgiques) | - |
dc.subject.classification | Càncer de pulmó | - |
dc.subject.classification | Proteïnes citosquelètiques | - |
dc.subject.classification | Micro RNAs | - |
dc.subject.other | Lung cancer | - |
dc.subject.other | Cytoskeletal proteins | - |
dc.subject.other | MicroRNAs | - |
dc.title | YKT6 expression, exosome release, and survival in non-small cell lung cancer | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 663235 | - |
dc.date.updated | 2017-01-02T16:55:41Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 27285987 | - |
Appears in Collections: | Articles publicats en revistes (Cirurgia i Especialitats Medicoquirúrgiques) Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) |
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File | Description | Size | Format | |
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663235.pdf | 2.41 MB | Adobe PDF | View/Open |
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