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dc.contributor.authorRuíz Martínez, Marc-
dc.contributor.authorNavarro Ponz, Alfons-
dc.contributor.authorMarrades Sicart, Ramon Ma.-
dc.contributor.authorViñolas Segarra, Núria-
dc.contributor.authorSantasusagna, Sandra-
dc.contributor.authorMuñoz García, Carmen-
dc.contributor.authorRamírez Ruz, J. (José)-
dc.contributor.authorMolins López-Rodó, Laureano-
dc.contributor.authorMonzó Planella, Mariano-
dc.description.abstractBACKGROUND: Cancer-derived exosomes are involved in metastasis. YKT6 is a SNARE protein that participates in the regulation of exosome production and release, but its role in non-small cell lung cancer (NSCLC) has not been examined. MATERIALS AND METHODS: Ultracentrifugation-purified exosomes from the A549 cell line were studied by CRYO-TEM, nanoparticle tracking analysis and western blot (TSG101 marker). YKT6 was inhibited using a DsiRNA and selected pre-microRNAs. MicroRNAs targeting YKT6 were validated by Renilla/Luciferase assay and western blot. YKT6 expression and its prognostic impact were analyzed in 98 tissue specimens from resected NSCLC patients. RESULTS: Membranous nanosized vesicles (mode size: 128nm) with TSG101 protein were purified from A549 cells. YKT6 inhibition reduced exosome release by 80.9%. We validated miR-134 and miR-135b as miRNAs targeting YKT6, and transfection with the pre-miRNAs also produced a significant reduction in exosome release. The analysis of YKT6 in tumor samples showed that patients with high levels had shorter disease-free and overall survival. CONCLUSIONS: YKT6 is a key molecule in the regulation of exosome release in lung cancer cells and is in turn precisely regulated by miR-134 and miR-135b. Moreover, YKT6 levels impact prognosis of resected NSCLC patients.-
dc.format.extent10 p.-
dc.publisherImpact Journals-
dc.relation.isformatofReproducció del document publicat a:
dc.relation.ispartofOncotarget, 2016, vol. 7, num. 32, p. 51515-51524-
dc.rightscc-by (c) Ruíz Martínez, Marc et al., 2016-
dc.sourceArticles publicats en revistes (Cirurgia i Especialitats Medicoquirúrgiques)-
dc.subject.classificationCàncer de pulmó-
dc.subject.classificationProteïnes citosquelètiques-
dc.subject.classificationMicro RNAs-
dc.subject.otherLung cancer-
dc.subject.otherCytoskeletal proteins-
dc.titleYKT6 expression, exosome release, and survival in non-small cell lung cancer-
Appears in Collections:Articles publicats en revistes (Cirurgia i Especialitats Medicoquirúrgiques)
Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)

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