Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/105206
Title: | YKT6 expression, exosome release, and survival in non-small cell lung cancer |
Author: | Ruíz Martínez, Marc Navarro Ponz, Alfons Marrades Sicart, Ramon Ma. Viñolas Segarra, Núria Santasusagna, Sandra Muñoz García, Carmen Ramírez Ruz, J. (José) Molins López-Rodó, Laureano Monzó Planella, Mariano |
Keywords: | Càncer de pulmó Proteïnes citosquelètiques Micro RNAs Lung cancer Cytoskeletal proteins MicroRNAs |
Issue Date: | 6-Jun-2016 |
Publisher: | Impact Journals |
Abstract: | BACKGROUND: Cancer-derived exosomes are involved in metastasis. YKT6 is a SNARE protein that participates in the regulation of exosome production and release, but its role in non-small cell lung cancer (NSCLC) has not been examined. MATERIALS AND METHODS: Ultracentrifugation-purified exosomes from the A549 cell line were studied by CRYO-TEM, nanoparticle tracking analysis and western blot (TSG101 marker). YKT6 was inhibited using a DsiRNA and selected pre-microRNAs. MicroRNAs targeting YKT6 were validated by Renilla/Luciferase assay and western blot. YKT6 expression and its prognostic impact were analyzed in 98 tissue specimens from resected NSCLC patients. RESULTS: Membranous nanosized vesicles (mode size: 128nm) with TSG101 protein were purified from A549 cells. YKT6 inhibition reduced exosome release by 80.9%. We validated miR-134 and miR-135b as miRNAs targeting YKT6, and transfection with the pre-miRNAs also produced a significant reduction in exosome release. The analysis of YKT6 in tumor samples showed that patients with high levels had shorter disease-free and overall survival. CONCLUSIONS: YKT6 is a key molecule in the regulation of exosome release in lung cancer cells and is in turn precisely regulated by miR-134 and miR-135b. Moreover, YKT6 levels impact prognosis of resected NSCLC patients. |
Note: | Reproducció del document publicat a: https://doi.org/10.18632/oncotarget.9862 |
It is part of: | Oncotarget, 2016, vol. 7, num. 32, p. 51515-51524 |
URI: | http://hdl.handle.net/2445/105206 |
Related resource: | https://doi.org/10.18632/oncotarget.9862 |
ISSN: | 1949-2553 |
Appears in Collections: | Articles publicats en revistes (Cirurgia i Especialitats Medicoquirúrgiques) Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) |
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