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Title: | Structural characterization of core Region in Erwinia amylovora lipopolysaccharide. |
Author: | Casillo, Angela Ziaco, Marcello Lindner, Buko Merino Montero, Susana Mendoza-Barberá, Elena Tomàs Magaña, Juan Corsaro, Maria Michela |
Keywords: | Bacteris patògens Genètica bacteriana Plantes Pathogenic bacteria Bacterial genetics Plants |
Issue Date: | 4-Mar-2017 |
Publisher: | MDPI |
Abstract: | Erwinia amylovora (E. amylovora) is the first bacterial plant pathogen described and demonstrated to cause fire blight, a devastating plant disease affecting a wide range of species including a wide variety of Rosaceae. In this study, we reported the lipopolysaccharide (LPS) core structure from E. amylovora strain CFBP1430, the first one for an E. amylovora highly pathogenic strain. The chemical characterization was performed on the mutants waaL (lacking only the O-antigen LPS with a complete LPS-core), wabH and wabG (outer-LPS core mutants). The LPSs were isolated from dry cells and analyzed by means of chemical and spectroscopic methods. In particular, they were subjected to a mild acid hydrolysis and/or a hydrazinolysis and investigated in detail by one and two dimensional Nuclear Magnetic Resonance (NMR) spectroscopy and ElectroSpray Ionization Fourier Transform-Ion Cyclotron Resonance (ESI FT-ICR) mass spectrometry. |
Note: | Reproducció del document publicat a: https://doi.org/10.3390/ijms18030559 |
It is part of: | International Journal of Molecular Sciences, 2017, vol. 18, num. 3, p. 559-559 |
URI: | http://hdl.handle.net/2445/109363 |
Related resource: | https://doi.org/10.3390/ijms18030559 |
ISSN: | 1422-0067 |
Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) |
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