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http://hdl.handle.net/2445/112611
Title: | The incC sequence is required for R27 plasmid stability |
Author: | Tassinari, Eleonora Aznar Vicente, Sonia Urcola, Imanol Prieto Durán, Alejandro Hüttener Queiroz, Mário Juárez Giménez, Antonio |
Keywords: | Seqüència d'aminoàcids Plasmidis Amino acid sequence Plasmids |
Issue Date: | 3-May-2016 |
Publisher: | Frontiers Media |
Abstract: | IncHI plasmids account for multiple antimicrobial resistance in Salmonella and other enterobacterial genera. These plasmids are generally very stable in their bacterial hosts. R27 is the archetype of IncHI1 plasmids. A high percentage of the R27-encoded open reading frames (ORFs) (66.7%) do not show similarity to any known ORFs. We performed a deletion analysis of all non-essential R27 DNA sequences to search for hitherto non-identified plasmid functions that might be required for plasmid stability. We report the identification of a short DNA sequence (incC) that is essential for R27 stability. That region contains several repeats (incC repeats), belongs to one of the three-plasmid replicons (R27 FIA-like) and is targeted by the R27 E protein. Deletion of the incC sequence drastically reduces R27 stability both in Escherichia coli and in Salmonella, the effect being more pronounced in this latter species. Interfering with incC-E protein interaction must lead to a reduced IncHI1 plasmid stability, and may represent a new approach to combat antimicrobial resistance. |
Note: | Reproducció del document publicat a: https://doi.org/10.3389/fmicb.2016.00629 |
It is part of: | Frontiers in Microbiology, 2016, vol. 7, num. 6, p. 629 |
URI: | http://hdl.handle.net/2445/112611 |
Related resource: | https://doi.org/10.3389/fmicb.2016.00629 |
ISSN: | 1664-302X |
Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC)) |
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