Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/116589
Title: | Improving virus production through quasispecies genomic selection and molecular breedings |
Author: | Pérez-Rodríguez, Francisco Javier D'Andrea Rodríguez-Vida, Lucía Castellarnau Serra, Montserrat de Costafreda Salvany, M. Isabel (Maria Isabel) Guix Arnau, Susana Ribes Mora, Enric Quer, Josep Gregori Font, Josep Bosch, Albert Pintó Solé, Rosa María |
Keywords: | Virologia Espècies (Biologia) Virology Species |
Issue Date: | 3-Nov-2016 |
Publisher: | Nature Publishing Group |
Abstract: | Virus production still is a challenging issue in antigen manufacture, particularly with slow-growing viruses. Deep-sequencing of genomic regions indicative of efficient replication may be used to identify high-fitness minority individuals suppressed by the ensemble of mutants in a virus quasispecies. Molecular breeding of quasispecies containing colonizer individuals, under regimes allowing more than one replicative cycle, is a strategy to select the fittest competitors among the colonizers. A slow-growing cell culture-adapted hepatitis A virus strain was employed as a model for this strategy. Using genomic selection in two regions predictive of efficient translation, the internal ribosome entry site and the VP1-coding region, high-fitness minority colonizer individuals were identified in a population adapted to conditions of artificially-induced cellular transcription shut-off. Molecular breeding of this population with a second one, also adapted to transcription shut-off and showing an overall colonizer phenotype, allowed the selection of a fast-growing population of great biotechnological potential. |
Note: | Reproducció del document publicat a: https://doi.org/10.1038/srep35962 |
It is part of: | Scientific Reports, 2016, vol. 6, p. 35962 |
URI: | http://hdl.handle.net/2445/116589 |
Related resource: | https://doi.org/10.1038/srep35962 |
ISSN: | 2045-2322 |
Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia) |
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