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http://hdl.handle.net/2445/117127
Title: | Atomic force microscopy visualization of injuries in Enterococcus faecalis surface caused by Er,Cr:YSGG and diode lasers |
Author: | López Jiménez, Lídia Arnabat Domínguez, Josep Viñas, Miquel Vinuesa Aumedes, Teresa |
Keywords: | Microscòpia de força atòmica Làsers Bacteris Atomic force microscopy Lasers Bacteria |
Issue Date: | Jan-2015 |
Publisher: | Medicina Oral SL |
Abstract: | Aim: To visualize by atomic force microscopy the alterations induced on Enterococcus. faecalis surface after treatment with 2 types of laser: Erbium chromium:yttrium-scandium-gallium-garnet (Er,Cr:YSGG) laser and Diode laser. Material and methods: Bacterial suspensions from overnight cultures of E. faecalis were irradiated during 30 seconds with the laser-lights at 1 W and 2 W of power, leaving one untreated sample as control. Surface alterations on treated E. faecalis were visualized by atomic force microscopy (AFM) and its surface roughness determined. Results: AFM imaging showed that at high potency of laser both cell morphology and surface roughness resulted altered, and that several cell lysis signs were easily visualized. Surface roughness clearly increase after the treatment with Er,Cr:YSGG at 2W of power, while the other treatments gave similar values of surface roughness. The effect of lasers on bacterial surfaces visualized by AFM revealed drastic alterations. Conclusions: AFM is a good tool to evaluate surface injuries after laser treatment; and could constitute a measure of antimicrobial effect that can complete data obtained by determination of microbial viability. |
Note: | Reproducció del document publicat a: https://doi.org/10.4317/medoral.19991 |
It is part of: | Medicina Oral, Patología Oral y Cirugia Bucal, 2015, vol. 20, num. 1, p. e45-e51 |
URI: | http://hdl.handle.net/2445/117127 |
Related resource: | https://doi.org/10.4317/medoral.19991 |
ISSN: | 1698-4447 |
Appears in Collections: | Articles publicats en revistes (Odontoestomatologia) Articles publicats en revistes (Patologia i Terapèutica Experimental) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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672485.pdf | 3.29 MB | Adobe PDF | View/Open |
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