Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/122309
Title: | Coaggregation, cointernalization, and codesensitization of adenosine A2A receptors and dopamine D2 receptors |
Author: | Hillion, Joëlle Canals Buj, Meritxell Torvinen, Maria Casadó, Vicent Scott, Rizaldy Terasmaa, Anton Hansson, Anita Watson, Stanley Olah, Mark E. Mallol Montero, Josefa Canela Campos, Enric I. Zoli, Michele Agnati, Luigi F. Ibanez, Carlos F. Lluís i Biset, Carme Franco Fernández, Rafael Ferré, Sergi Fuxe, Kjell |
Keywords: | Receptors cel·lulars Malalties del sistema nerviós Adenosina Cell receptors Nervous system Diseases Adenosine |
Issue Date: | May-2002 |
Publisher: | American Society for Biochemistry and Molecular Biology |
Abstract: | Antagonistic and reciprocal interactions are known to exist between adenosine and dopamine receptors in the striatum. In the present study, double immunofluorescence experiments with confocal laser microscopy showed a high degree of colocalization of adenosine A(2A) receptors (A(2A)R) and dopamine D(2) receptors (D(2)R) in cell membranes of SH-SY5Y human neuroblastoma cells stably transfected with human D(2)R and in cultured striatal cells. A(2A)R/D(2)R heteromeric complexes were demonstrated in coimmunoprecipitation experiments in membrane preparations from D(2)R-transfected SH-SY5Y cells and from mouse fibroblast Ltk(-) cells stably transfected with human D(2)R (long form) and transiently cotransfected with the A(2A)R double-tagged with hemagglutinin. Long term exposure to A(2A)R and D(2)R agonists in D(2)R-cotransfected SH-SY5Y cells resulted in coaggregation, cointernalization and codesensitization of A(2A)R and D(2)R. These results give a molecular basis for adenosine-dopamine antagonism at the membrane level and have implications for treatment of Parkinson's disease and schizophrenia, in which D(2)R are involved. |
Note: | Reproducció del document publicat a: https://doi.org/10.1074/jbc.M107731200 |
It is part of: | Journal of Biological Chemistry, 2002, vol. 277, num. 20, p. 18091-18097 |
URI: | http://hdl.handle.net/2445/122309 |
Related resource: | https://doi.org/10.1074/jbc.M107731200 |
ISSN: | 0021-9258 |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Biomedicina Molecular) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
501595.pdf | 607.29 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.