Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/122383
Title: | Actin-binding protein α-actinin-1 interacts with the metabotropic glutamate receptor type 5b and modulates the cell surface expression and function of the receptor |
Author: | Cabello, Nuria Remelli, Rosaria Canela, Laia Soriguera, Ana Mallol Montero, Josefa Canela Campos, Enric I. Robbins, Melanie J. Lluís i Biset, Carme Franco Fernández, Rafael McIlhinney, R. A. Jeffrey Ciruela Alférez, Francisco |
Keywords: | Àcid glutàmic Neurotransmissió Glutamic acid Neural transmission |
Issue Date: | 20-Apr-2007 |
Publisher: | American Society for Biochemistry and Molecular Biology |
Abstract: | Receptors for neurotransmitters require scaffolding proteins for membrane microdomain targeting and for regulating receptor function. Using a yeast two-hybrid screen, α-actinin-1, a major F-actin cross-linking protein, was identified as a binding partner for the C-terminal domain of metabotropic glutamate receptor type 5b (mGlu5b receptor). Co-expression, co-immunoprecipitation, and pull-down experiments showed a close and specific interaction between mGlu5b receptor and α-actinin-1 in both transfected HEK-293 cells and rat striatum. The interaction of α-actinin-1 with mGlu5b receptor modulated the cell surface expression of the receptor. This was dependent on the binding of α-actinin-1 to the actin cytoskeleton. In addition, the α-actinin-1/mGlu5b receptor interaction regulated receptor-mediated activation of the mitogen-activated protein kinase pathway. Together, these findings indicate that there is an α-actinin-1-dependent mGlu5b receptor association with the actin cytoskeleton modulating receptor cell surface expression and functioning. |
Note: | Reproducció del document publicat a: https://doi.org/10.1074/jbc.M608880200 |
It is part of: | Journal of Biological Chemistry, 2007, vol. 282, num. 16, p. 12143-12153 |
URI: | http://hdl.handle.net/2445/122383 |
Related resource: | https://doi.org/10.1074/jbc.M608880200 |
ISSN: | 0021-9258 |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Biomedicina Molecular) Articles publicats en revistes (Patologia i Terapèutica Experimental) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
549463.pdf | 607.37 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.