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Title: | Is Penicillin Plus Gentamicin Synergistic Against Sessile Group B Streptococcal Isolates? An In Vivo Study With An Experimental Model Of Foreign-body Infection |
Author: | Ruppen, Corinne Mercier, Thomas Grandgirard, Denis Leib, Sthephen L. El Haj Hidalgo, Cristina Murillo Rubio, Óscar Decosterd, Laurent Sendi, Parham |
Keywords: | Estreptococs Penicil·lina Biofilms Streptococcus Penicillin Biofilms |
Issue Date: | 15-May-2018 |
Publisher: | Frontiers Media Sa |
Abstract: | The rate of invasive group B Streptococcus (GBS) infections is steadily increasing, particularly in older persons and in adults with diabetes and other comorbidities. This population includes persons with a foreign body (e.g., who have undergone arthroplasty). In a rat tissue cage model, we evaluated the efficacy of adjunctive gentamicin (GEN) administered systemically (5 mg/kg body weight) every 24 h, or locally (12.5 mg/L tissue cage concentration) every 24 or 72 h, in combination with penicillin (PEN) administered systemically (250,000 IU/kg body weight three times per day). The efficacy was evaluated on two different sessile forms of GBS: transition (i.e., in between planktonic and biofilm) and biofilm. After 3 days of treatment, the mean bacterial load reduction of transition-form GBS was greater in all PEN-GEN combination groups than in the PEN monotherapy group (P <= 0.03). The 6-day regimen decreased the bacterial load significantly in comparison to the 3-day regimen, irrespective of growth form and adjunctive GEN (P < 0.01). After 6 days of treatment, the mean reduction in transition-form GBS was greater with PEN plus GEN administered locally every 24 h than with PEN monotherapy (P = 0.03). These results were not confirmed with biofilm GBS. The difference in mean bacterial load reduction between all PEN-GEN and PEN monotherapy groups was <100 CFU/mL. Hence, synergy criteria were not fulfilled. Adjunctive systemic GEN consists of potential side effects and showed poor efficacy in this study. Combining systemic PEN and local GEN has a potential application in the treatment of streptococcal implant-associated infections. |
Note: | Reproducció del document publicat a: https://doi.org/10.3389/fmicb.2018.00919 |
It is part of: | Frontiers In Microbiology, 2018, Vol. 9, article 919 |
URI: | http://hdl.handle.net/2445/123978 |
Related resource: | https://doi.org/10.3389/fmicb.2018.00919 |
Appears in Collections: | Articles publicats en revistes (Ciències Clíniques) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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