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Title: | Cholesterol depletion in adipocytes causes caveolae collapse concomitant with proteosomal degradation of cavin-2 in a switch-like fashion |
Author: | Breen, Michael P., 1940- Camps Camprubí, Marta Carvalho-Simoes, Francisco Zorzano Olarte, Antonio Pilch, Paul F. |
Keywords: | Colesterol Proteïnes de membrana Cèl·lules animals Cholesterol Membrane proteins Animal cells |
Issue Date: | 6-Apr-2012 |
Publisher: | Public Library of Science (PLoS) |
Abstract: | Caveolae, little caves of cell surfaces, are enriched in cholesterol, a certain level of which is required for their structural integrity. Here we show in adipocytes that cavin-2, a peripheral membrane protein and one of 3 cavin isoforms present in caveolae from non-muscle tissue, is degraded upon cholesterol depletion in a rapid fashion resulting in collapse of caveolae. We exposed 3T3-L1 adipocytes to the cholesterol depleting agent methyl-β-cyclodextrin, which results in a sudden and extensive degradation of cavin-2 by the proteasome and a concomitant movement of cavin-1 from the plasma membrane to the cytosol along with loss of caveolae. The recovery of cavin-2 at the plasma membrane is cholesterol-dependent and is required for the return of cavin-1 from the cytosol to the cell surface and caveolae restoration. Expression of shRNA directed against cavin-2 also results in a cytosolic distribution of cavin-1 and loss of caveolae. Taken together, these data demonstrate that cavin-2 functions as a cholesterol responsive component of caveolae that is required for cavin-1 localization to the plasma membrane, and caveolae structural integrity. |
Note: | Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0034516 |
It is part of: | PLoS One, 2012, vol. 7, num. 4, p. 1-8 |
URI: | http://hdl.handle.net/2445/124628 |
Related resource: | https://doi.org/10.1371/journal.pone.0034516 |
ISSN: | 1932-6203 |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Biomedicina Molecular) |
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