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http://hdl.handle.net/2445/125691
Title: | Untangling dopamine-adenosine receptor assembly in experimental parkinsonism in rats |
Author: | Fernández Dueñas, Víctor Taura, Jaume Cottet, Martin Gómez Soler, Maricel López-Cano, Marc Ledent, Catherine Watanabe, Masahiko Trinquet, Eric Pin, Jean-Philippe Luján, Rafael Durroux, Thierry Ciruela Alférez, Francisco |
Keywords: | Malaltia de Parkinson Adenosina Dopamina Parkinson's disease Adenosine Dopamine |
Issue Date: | 1-Jan-2015 |
Publisher: | The Company of Biologists |
Abstract: | Parkinson's disease (PD) is a dopaminergic-related pathology in which functioning of the basal ganglia is altered. It has been postulated that a direct receptor-receptor interaction - i.e. of dopamine D-2 receptor (D2R) with adenosine A(2A) receptor (A(2A)R) (forming D2R-A(2A)R oligomers) - finely regulates this brain area. Accordingly, elucidating whether the pathology prompts changes to these complexes could provide valuable information for the design of new PD therapies. Here, we first resolved a long-standing question concerning whether D2R-A(2A)R assembly occurs in native tissue: by means of different complementary experimental approaches (i.e. immunoelectron microscopy, proximity ligation assay and TR-FRET), we unambiguously identified native D2R-A(2A)R oligomers in rat striatum. Subsequently, we determined that, under pathological conditions (i.e. in a rat PD model), D2R-A(2A)R interaction was impaired. Collectively, these results provide definitive evidence for alteration of native D2R-A(2A)R oligomers in experimental parkinsonism, thus conferring the rationale for appropriate oligomer-based PD treatments. |
Note: | Reproducció del document publicat a: https://doi.org/10.1242/dmm.018143 |
It is part of: | Disease Models & Mechanisms, 2015, vol. 8, num. 1, p. 57-63 |
URI: | http://hdl.handle.net/2445/125691 |
Related resource: | https://doi.org/10.1242/dmm.018143 |
ISSN: | 1754-8403 |
Appears in Collections: | Articles publicats en revistes (Patologia i Terapèutica Experimental) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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