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http://hdl.handle.net/2445/125974
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DC Field | Value | Language |
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dc.contributor.author | Rodríguez-Rubio, Lorena | - |
dc.contributor.author | Chang, Wai-Ling | - |
dc.contributor.author | Gutiérrez, Diana | - |
dc.contributor.author | Lavigne, Rob | - |
dc.contributor.author | Martínez, Beatriz | - |
dc.contributor.author | Rodríguez, Ana | - |
dc.contributor.author | Govers, Sander K. | - |
dc.contributor.author | Aertsen, Abram | - |
dc.contributor.author | Hirl, Christine | - |
dc.contributor.author | Biebl, Manfred | - |
dc.contributor.author | Briers, Yves | - |
dc.contributor.author | García, Pilar | - |
dc.date.accessioned | 2018-11-09T16:15:56Z | - |
dc.date.available | 2018-11-09T16:15:56Z | - |
dc.date.issued | 2016-10-24 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://hdl.handle.net/2445/125974 | - |
dc.description.abstract | Endolysins constitute a promising class of antibacterials against Gram-positive bacteria. Recently, endolysins have been engineered with selected peptides to obtain a new generation of lytic proteins, Artilysins, with specific activity against Gram-negative bacteria. Here, we demonstrate that artilysation can also be used to enhance the antibacterial activity of endolysins against Gram-positive bacteria and to reduce the dependence on external conditions. Art-240, a chimeric protein of the anti-streptococcal endolysin λSa2lys and the polycationic peptide PCNP, shows a similar species specificity as the parental endolysin, but the bactericidal activity against streptococci increases and is less affected by elevated NaCl concentrations and pH variations. Time-kill experiments and time-lapse microscopy demonstrate that the killing rate of Art-240 is approximately two-fold higher compared to wildtype endolysin λSa2lys, with a reduction in viable bacteria of 3 log units after 10min. In addition, lower doses of Art240 are required to achieve the same bactericidal effect. | - |
dc.format.extent | 11 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Nature Publishing Group | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1038/srep35382 | - |
dc.relation.ispartof | Scientific Reports, 2016, vol. 6, num. 35382 | - |
dc.relation.uri | https://doi.org/10.1038/srep35382 | - |
dc.rights | cc-by (c) Rodriguez Rubio, Lorena et al., 2016 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Genètica, Microbiologia i Estadística) | - |
dc.subject.classification | Estreptococs | - |
dc.subject.classification | Enzims microbians | - |
dc.subject.classification | Bacteriòfags | - |
dc.subject.other | Streptococcus | - |
dc.subject.other | Microbial enzymes | - |
dc.subject.other | Bacteriophages | - |
dc.title | Artilysation' of endolysin λSa2lys strongly improves its enzymatic and antibacterial activity against streptococci | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 680685 | - |
dc.date.updated | 2018-11-09T16:15:56Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 27775093 | - |
Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) |
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File | Description | Size | Format | |
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680685.pdf | 1.44 MB | Adobe PDF | View/Open |
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