Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/126429
Title: | Lighting up multiprotein complexes: lessons from GPCR oligomerization |
Author: | Ciruela Alférez, Francisco Vilardaga, Jean Pierre Fernández Dueñas, Víctor |
Keywords: | Proteïnes Oligòmers Proteins Oligomers |
Issue Date: | Aug-2010 |
Publisher: | Elsevier Current Trends |
Abstract: | Spatiotemporal characterization of protein protein interactions (PPIs) is essential in determining the molecular mechanisms of intracellular signaling processes. In this review, we discuss how new methodological strategies derived from non-invasive fluorescence- and luminescence-based approaches (FRET, BRET, BiFC and BiLC), when applied to the study of G protein-coupled receptor (GPCR) oligomerization, can be used to detect specific PPIs in live cells. These technologies alone or in concert with complementary methods (SRET, BRET or BiFC, and SNAP-tag or TR-FRET) can be extremely powerful approaches for PPI visualization, even between more than two proteins. Here we provide a comprehensive update on all the biotechnological aspects, including the strengths and weaknesses, of new fluorescence- and luminescence-based methodologies, with a specific focus on their application for studying PPIs. |
Note: | Versió postprint del document publicat a: https://doi.org/10.1016/j.tibtech.2010.05.002 |
It is part of: | Trends in Biotechnology, 2010, vol. 28, num. 8, p. 407-415 |
URI: | http://hdl.handle.net/2445/126429 |
Related resource: | https://doi.org/10.1016/j.tibtech.2010.05.002 |
ISSN: | 0167-7799 |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) Articles publicats en revistes (Patologia i Terapèutica Experimental) |
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