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http://hdl.handle.net/2445/127854
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DC Field | Value | Language |
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dc.contributor.author | Santas Gutiérrez, Jonathan | - |
dc.contributor.author | Guardiola Ibarz, Francesc | - |
dc.contributor.author | Rafecas Martínez, Magdalena | - |
dc.contributor.author | Bou Novensà, Ricard | - |
dc.date.accessioned | 2019-02-04T13:08:53Z | - |
dc.date.available | 2019-02-04T13:08:53Z | - |
dc.date.issued | 2013-03-01 | - |
dc.identifier.issn | 0003-2697 | - |
dc.identifier.uri | http://hdl.handle.net/2445/127854 | - |
dc.description.abstract | Plasma hydroperoxides (HPs) are widely accepted to be good indicators of oxidative stress. By means of the method proposed here, which uses diphenyl-1-pyrenylphosphine (DPPP) as a fluorescent probe, all types of plasma HP were determined. The limits of detection and quantification of the method were 0.08 and 0.25 nmol of cumene hydroperoxide (CHP) equivalents in 40 μl of plasma, respectively. The method is satisfactory in terms of precision (5.3% for 14.5 μM CHP eq., n=8), and the recoveries were 91% and 92% after standard additions of 26 and 52 μM CHP, respectively. The selectivity of the proposed method is higher than 96%. Moreover, optimization of the reaction conditions and the addition of ethylenediaminetetraacetic acid (EDTA) disodium salt and 2,6-di-tert-butyl-4-methylphenol (BHT) prevented the formation of HP artifacts during the analysis. Therefore, the proposed method is useful for simple and quantitative determination of total plasma HPs. | - |
dc.format.extent | 6 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier | - |
dc.relation.isformatof | Versió postprint del document publicat a: https://doi.org/10.1016/j.ab.2012.11.021 | - |
dc.relation.ispartof | Analytical Biochemistry, 2013, vol. 434, num. 1, p. 172-177 | - |
dc.relation.uri | https://doi.org/10.1016/j.ab.2012.11.021 | - |
dc.rights | (c) Elsevier, 2013 | - |
dc.source | Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia) | - |
dc.subject.classification | Estrès oxidatiu | - |
dc.subject.classification | Plasma sanguini | - |
dc.subject.classification | Química analítica | - |
dc.subject.classification | Mètodes experimentals | - |
dc.subject.classification | Cromatografia de líquids d'alta resolució | - |
dc.subject.other | Oxidative stress | - |
dc.subject.other | Blood plasma | - |
dc.subject.other | Analytical chemistry | - |
dc.subject.other | Experimental methods | - |
dc.subject.other | High performance liquid chromatography | - |
dc.title | Determination of total plasma hydroperoxides using a diphenyl-1-pyrenylphosphine fluorescent probe | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 618562 | - |
dc.date.updated | 2019-02-04T13:08:53Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia) |
Files in This Item:
File | Description | Size | Format | |
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618562.pdf | 244.92 kB | Adobe PDF | View/Open |
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