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Title: Development and validation of a high performance liquid chromatography method to determine linezolid concentrations in pig pulmonary tissue
Author: Guerrero Latorre, Laura
Martínez-Olondris, Pilar
Rigol, Montserrat
Esperatti, Mariano
Esquinas López, Cristina
Luque Chipana, Néstor Alejandro
Piñer, Raquel
Torres Martí, Antoni
Soy Muner, Dolors
Keywords: Anàlisi
Agents antiinfecciosos
Cromatografia de líquids d'alta resolució
Compostos heterocíclics
Anti-infective agents
High performance liquid chromatography
Heterocyclic compounds
Issue Date: 2010
Publisher: Walter de Gruyter
Abstract: Background: linezolid is the first synthetic compound of a new group of antimicrobials, the oxazolidinones, which inhibit protein synthesis. It shows a broad spectrum of activity against Gram positive organisms. With respect to its pharmacokinetics, linezolid shows a relatively high volume of distribution and good penetration into inflammatory fluids, bone, fat and muscle. Methods: a reversed-phase isocratic high-performance liquid chromatographic method for linezolid analysis in piglet pulmonary tissue is described. Tissue samples and controls were prepared in 1 x TBE (1 M Tris, 0.9 M boric acid, 0.01 M EDTA). The mobile phase consisted of 20% ultrafiltered water and 80% of (A) 15 mM potassium monohydrogen phosphate buffer (pH = 5) with (B) acetonitrile (80%/20%; v/v). Samples were homogenized and precipitated with HClO(4) 3% (1/1, v/v). The injection volume was 100 microL. Ofloxacin was used as an internal standard. Results: the assay was linear over a linezolid concentration range: 1.6-100 microg/mL. The method provided good validation data (n = 15): inaccuracy (3.6%), intra and inter-day variability (4.2% and 5.2%, respectively), recovery (91.8%), limit of detection (0.8 microg/mL) and quantitation (1.6 microg/mL) and acceptable stability within 24 h in the auto-sampler. Conclusions: the method offers a fast and simple approach to determine linezolid in pulmonary tissue which could be of use in pharmacokinetic studies.
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It is part of: Clinical Chemistry and Laboratory Medicine, 2010, vol. 3, num. 48, p. 391-398
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ISSN: 1434-6621
Appears in Collections:Articles publicats en revistes (Genètica, Microbiologia i Estadística)
Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)
Articles publicats en revistes (Infermeria de Salut Pública, Salut mental i Maternoinfantil)
Articles publicats en revistes (Medicina)

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