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Title: | A Plasmodium falciparum C-mannosyltransferase is dispensable for parasite asexual blood stage development |
Author: | López Gutiérrez, Borja Cova, Marta Izquierdo Lázaro, Luis |
Keywords: | Plasmodium falciparum Paràsits Parasites |
Issue Date: | 27-Sep-2019 |
Publisher: | Cambridge University Press |
Abstract: | C-mannosylation was recently identified in the thrombospondin-related anonymous protein (TRAP) from Plasmodium falciparum salivary gland sporozoites. A candidate P. falciparum C-mannosyltransferase (Pf DPY-19) was demonstrated to modify thrombospondin type 1 repeat (TSR) domains in vitro, exhibiting a different acceptor specificity than their mammalian counterparts. According to the described minimal acceptor of Pf DPY19, several TSR domain-containing proteins of P. falciparum could be C-mannosylated in vivo. However, the relevance of this protein modification for the parasite viability remains unknown. In the present study, we used CRISPR/Cas9 technology to generate a Pf DPY19 null mutant, demonstrating that this glycosyltransferase is not essential for the asexual blood development of the parasite. Pf DPY19 gene disruption was not associated with a growth phenotype, not even under endoplasmic reticulum-stressing conditions that could impair protein folding. The data presented in this work strongly suggest that Pf DPY19 is unlikely to play a critical role in the asexual blood stages of the parasite, at least under in vitro conditions. |
Note: | Reproducció del document publicat a: http://dx.doi.org/10.1017/S0031182019001380 |
It is part of: | Parasitology, 2019 |
URI: | http://hdl.handle.net/2445/143332 |
Related resource: | http://dx.doi.org/10.1017/S0031182019001380 |
ISSN: | 0031-1820 |
Appears in Collections: | Articles publicats en revistes (ISGlobal) |
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