Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/148840
Title: | Structural insights into “acid blobs and negative noodles” – The androgen receptor as a case study |
Author: | Szulc, Elzbieta Maria |
Director/Tutor: | Salvatella i Giralt, Xavier |
Keywords: | Factors de transcripció Càncer de pròstata Andrògens Proteïnes Transcription factors Prostate cancer Androgens Proteins |
Issue Date: | 9-Jan-2020 |
Publisher: | Universitat de Barcelona |
Abstract: | [eng] Androgen receptor is a transcription factor that plays a key role in prostate cancer development and progression. Its N-terminal intrinsically disordered activation domain is an example of ill- structured negatively charged "acid blob" or "negative noodle" described in 1988 by Paul B. Sigler. In his model the transcription pre-initiation complex formation relies on non-specific interactions between the "negative noodles" of the transcriptional activating proteins and hydroxyl groups of the heptapeptide repeats of the C-terminal domain in the largest subunit of RNA polymerase II. Here, we take advantage of various biophysical methods to understand the AR activation domain characteristics, its ability to undergo liquid-liquid phase separation and the importance of both for protein-protein interactions of the domain. We show relevance of the secondary structure elements in the AD function despite its general "acid blob" character. |
URI: | http://hdl.handle.net/2445/148840 |
Appears in Collections: | Tesis Doctorals - Facultat - Farmàcia i Ciències de l'Alimentació |
Files in This Item:
File | Description | Size | Format | |
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EMS_PhD_THESIS.pdf | 14.08 MB | Adobe PDF | View/Open |
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