Please use this identifier to cite or link to this item:
https://hdl.handle.net/2445/151389
Title: | Identification of antihypertensive peptides in nutraceuticals by capillary electrophoresis-mass spectrometry |
Author: | Benavente Moreno, Fernando J. (Julián) Peró Gascón, Roger Pont Villanueva, Laura Jaumot Soler, Joaquim Barbosa Torralbo, José Sanz Nebot, María Victoria |
Keywords: | Pèptids Electroforesi capil·lar Quimiometria Espectrometria de masses Peptides Capillary electrophoresis Chemometrics Mass spectrometry |
Issue Date: | 2018 |
Publisher: | Elsevier B.V. |
Abstract: | We present capillary electrophoresis-mass spectrometry (CE-MS) in combination with advanced chemometric tools for the analysis of bioactive compounds in food, in particular for the identification of antihypertensive peptides in a nutraceutical derived from a bovine milk protein hydrolysate. Different extracts of the nutraceutical were analyzed by CE-MS, and the electropherograms were processed using a novel data analysis workflow that included regions of interest (ROIs) compression and multivariate curve resolution alternating least squares (MCR-ALS). MCR-ALS permitted the description of the nutraceutical extract as ten characteristic components with their electrophoretic profiles and mass spectra. Twenty-two compounds were tentatively identified as antihypertensive bovine casein fragments through a mass search in a database of bioactive peptides. The identity of 17 antihypertensive peptides was reliably confirmed by capillary electrophoresis-tandem mass spectrometry. The proposed analytical approach demonstrated the potential to obtain a characteristic and activity-related fingerprint for quality control and authentication of the antihypertensive nutraceutical. |
Note: | Versió postprint del document publicat a: https://doi.org/10.1016/j.chroma.2018.10.018 |
It is part of: | Journal of Chromatography A, 2018, vol. 1579, p. 129-137 |
URI: | https://hdl.handle.net/2445/151389 |
Related resource: | https://doi.org/10.1016/j.chroma.2018.10.018 |
ISSN: | 0021-9673 |
Appears in Collections: | Articles publicats en revistes (Enginyeria Química i Química Analítica) |
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