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http://hdl.handle.net/2445/151684
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DC Field | Value | Language |
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dc.contributor.author | Hrvolová, Barbora | - |
dc.contributor.author | Colmán Martínez, Lourdes Mariel | - |
dc.contributor.author | Martínez Huélamo, Miriam | - |
dc.contributor.author | Hurtado Barroso, Sara | - |
dc.contributor.author | Lamuela Raventós, Rosa Ma. | - |
dc.contributor.author | Kalina, Jirí | - |
dc.date.accessioned | 2020-03-02T17:16:55Z | - |
dc.date.available | 2020-03-02T17:16:55Z | - |
dc.date.issued | 2016-10-14 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | http://hdl.handle.net/2445/151684 | - |
dc.description.abstract | The concentration of carotenoids and fat-soluble vitamins in human plasma may play a significant role in numerous chronic diseases such as age-related macular degeneration and some types of cancer. Although these compounds are of utmost interest for human health, methods for their simultaneous determination are scarce. A new high pressure liquid chromatography (HPLC)-tandem mass spectrometry (MS/MS) method for the quantification of selected carotenoids and fat-soluble vitamins in human plasma was developed, validated, and then applied in a pilot dietary intervention study with healthy volunteers. In 50 min, 16 analytes were separated with an excellent resolution and suitable MS signal intensity. The proposed HPLC-MS/MS method led to improvements in the limits of detection (LOD) and quantification (LOQ) for all analyzed compounds compared to the most often used HPLC-DAD methods, in some cases being more than 100-fold lower. LOD values were between 0.001 and 0.422 µg/mL and LOQ values ranged from 0.003 to 1.406 µg/mL, according to the analyte. The accuracy, precision, and stability met with the acceptance criteria of the AOAC (Association of Official Analytical Chemists) International. According to these results, the described HPLC-MS/MS method is adequately sensitive, repeatable and suitable for the large-scale analysis of compounds in biological fluids. | - |
dc.format.extent | 17 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | MDPI | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.3390/ijms17101719 | - |
dc.relation.ispartof | International Journal of Molecular Sciences, 2016, vol. 17, num. 10, p. E1719 | - |
dc.relation.uri | https://doi.org/10.3390/ijms17101719 | - |
dc.rights | cc-by (c) Hrvolová, Barbora et al., 2016 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia) | - |
dc.subject.classification | Carotenoides | - |
dc.subject.classification | Vitamines en la nutrició humana | - |
dc.subject.classification | Plasma sanguini | - |
dc.subject.other | Carotenoids | - |
dc.subject.other | Vitamins in human nutrition | - |
dc.subject.other | Blood plasma | - |
dc.title | Development of an advanced HPLC-MS/MS method for the determination of carotenoids and fat-soluble vitamins in human plasma | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 668871 | - |
dc.date.updated | 2020-03-02T17:16:55Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 27754400 | - |
Appears in Collections: | Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia) |
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668871.pdf | 932.39 kB | Adobe PDF | View/Open |
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