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Title: | Rainbow trout slow myoblast cell culture as a model to study slow skeletal muscle and the characterization of mir-133 and mir-499 families as a case study |
Author: | Duran, Bruno Oliveira da Silva Dal-Pai-Silva, Maeli García de la Serrana Castillo, Daniel |
Keywords: | Esquelet humà Fibres Músculs Human skeleton Fibers Muscles |
Issue Date: | 23-Dec-2019 |
Publisher: | The Company of Biologists |
Abstract: | Muscle fibres are classified as fast, intermediate and slow. In vitro myoblast cell culture model from fast muscle is a very useful tool to study muscle growth and development; however, similar models for slow muscle do not exist. Owing to the compartmentalization of fish muscle fibres, we have developed a slow myoblast cell culture for rainbow trout (Oncorhynchus mykiss). Slow and fast muscle-derived myoblasts have similar morphology, but with differential expression of slow muscle markers such as slow myhc, sox6 and pgc-1α. We also characterized the mir-133 and mir-499 microRNA families in trout slow and fast myoblasts as a case study during myogenesis and in response to electrostimulation. Three mir-133 (a-1a, a-1b and a-2) and four mir-499 (aa, ab, ba and bb) paralogues were identified for rainbow trout and named base on their phylogenetic relationship to zebrafish and Atlantic salmon orthologues. Omy-mir-499ab and omy-mir-499bb had 0.6 and 0.5-fold higher expression in slow myoblasts compared with fast myoblasts, whereas mir-133 duplicates had similar levels in both phenotypes and little variation during development. Slow myoblasts also showed increased expression for omy-mir-499b paralogues in response to chronic electrostimulation (7-fold increase for omy-mir-499ba and 2.5-fold increase for omy-mir-499bb). The higher expression of mir-499 paralogues in slow myoblasts suggests a role in phenotype determination, while the lack of significant differences of mir-133 copies during culture development might indicate a different role in fish compared with mammals. We have also found signs of sub-functionalization of mir-499 paralogues after electrostimulation, with omy-mir-499b copies more responsive to electrical signals. |
Note: | Reproducció del document publicat a: https://doi.org/10.1242/jeb.216390 |
It is part of: | Journal of Experimental Biology, 2019, vol. 223, p. 216390 |
URI: | http://hdl.handle.net/2445/151827 |
Related resource: | https://doi.org/10.1242/jeb.216390 |
ISSN: | 0022-0949 |
Appears in Collections: | Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia) |
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