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http://hdl.handle.net/2445/152738
Title: | Sensing of nutrients by CPT1C regulates late endosome/lysosome anterograde transport and axon growth |
Author: | Palomo-Guerrero, Marta Fadó Andrés, Rut Casas, Maria Pérez-Montero, Marta Baena Muñoz, Miguel Helmer, Patrick O Domínguez, José Luis Roig, Aina Serra i Cucurull, Dolors Hayen, Heiko Stenmark, Harald Raiborg, Camilla Casals, Núria |
Keywords: | Carnitina palmitoïl-transferasa 1 Axons Lisosomes Carnitine palmitoyltransferase I Axons Lysosomes |
Issue Date: | 23-Dec-2019 |
Publisher: | eLife Sciences |
Abstract: | Anterograde transport of late endosomes or lysosomes (LE/Lys) is crucial for proper axon growth. However, the role of energetic nutrients has been poorly explored. Malonyl-CoA is a precursor of fatty acids, and its intracellular levels highly fluctuate depending on glucose availability or the energy sensor AMP-activated protein kinase (AMPK). We demonstrate in HeLa cells that carnitine palmitoyltransferase 1C (CPT1C) senses malonyl-CoA and enhances LE/Lys anterograde transport by interacting with the endoplasmic reticulum protein protrudin and facilitating the transfer of Kinesin-1 from protrudin to LE/Lys. In cultured mouse cortical neurons, glucose deprivation, pharmacological activation of AMPK or inhibition of malonyl-CoA synthesis decreases LE/Lys abundance at the axon terminal, and shortens axon length in a CPT1C-dependent manner. These results identify CPT1C as a new regulator of anterograde LE/Lys transport in response to malonyl-CoA changes, and give insight into how axon growth is controlled by nutrients. |
Note: | Reproducció del document publicat a: https://doi.org/10.7554/eLife.51063 |
It is part of: | eLife, 2019, vol. 8, p. e51063 |
URI: | http://hdl.handle.net/2445/152738 |
Related resource: | https://doi.org/10.7554/eLife.51063 |
ISSN: | 2050-084X |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Fisiologia) |
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