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http://hdl.handle.net/2445/159057
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DC Field | Value | Language |
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dc.contributor.author | De-Ugarte, Laura | - |
dc.contributor.author | Balcells Comas, Susana | - |
dc.contributor.author | Güerri Fernández, Robert | - |
dc.contributor.author | Grinberg Vaisman, Daniel Raúl | - |
dc.contributor.author | Diez-Perez, Adolfo | - |
dc.contributor.author | Nogués Solán, Xavier | - |
dc.contributor.author | Garcia Giralt, Natàlia | - |
dc.date.accessioned | 2020-05-07T07:37:31Z | - |
dc.date.available | 2020-05-07T07:37:31Z | - |
dc.date.issued | 2020-04-20 | - |
dc.identifier.issn | 2076-3417 | - |
dc.identifier.uri | http://hdl.handle.net/2445/159057 | - |
dc.description.abstract | The miR-320a regulates a number of genes involved in various physiological processes. In particular, it has been reported as a tumor suppressor in several types of human cancers and involved in osteoporotic fracture and osteoblast function. Hence, the role of miR-320a has been evaluated in tumor cells and in primary cells in a separated context, but its effect has never been explored in a comparative manner. The present study aims to evaluate the cellular effects of miR-320a on human osteosarcoma cell lines (MG-63 and U2OS) compared to that on primary human osteoblasts (hOBs). miR-320a was either overexpressed or inhibited in all cell lines, and cell proliferation and viability were analyzed. Additionally, the effects of miR-320a on matrix mineralization, alkaline phosphatase activity, and oxidative stress were also evaluated in order to assess osteoblast functionality. In osteosarcoma cells, miR-320a overexpression reduced cell viability and proliferation, while in hOB cell viability was not affected and proliferation even was increased. The overexpression of miR-320a in both osteosarcoma cells and hOBs reduced the mineralization capacity. Finally, an increased oxidative stress was detected in all cells after miR-320a overexpression mainly in osteosarcoma. In conclusion, the overexpression of miR-320a increased stress oxidation levels, which could be involved in the reduced osteoblast performance, even though the cell viability was only affected in osteosarcoma cells. | - |
dc.format.extent | 11 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | MDPI | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.3390/app10082852 | - |
dc.relation.ispartof | Applied Sciences, 2020, vol. 10, p. 2852 | - |
dc.relation.uri | https://doi.org/10.3390/app10082852 | - |
dc.rights | cc-by (c) De-Ugarte, Laura et al., 2020 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Genètica, Microbiologia i Estadística) | - |
dc.subject.classification | Osteosarcoma | - |
dc.subject.other | Osteosarcoma | - |
dc.title | Effect of the tumor suppressor miR-320a on viability and functionality of human osteosarcoma cell lines compared to primary osteoblasts | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 699025 | - |
dc.date.updated | 2020-05-07T07:37:31Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) |
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699025.pdf | 998.81 kB | Adobe PDF | View/Open |
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