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Title: | Enrichment of histidine containing peptides by on-line immobilized metal affinity solid-phase extraction capillary electrophoresis-mass spectrometry |
Author: | Peró Gascón, Roger Giménez López, Estela Sanz Nebot, María Victoria Benavente Moreno, Fernando J. (Julián) |
Keywords: | Electroforesi capil·lar Espectrometria de masses Pèptids Capillary electrophoresis Mass spectrometry Peptides |
Issue Date: | 12-May-2020 |
Publisher: | Elsevier B.V. |
Abstract: | The analysis and detection of targeted peptides has received due attention in many fields of proteomics research, such as discovery of biomarkers. In this regard, capillary electrophoresis-mass spectrometry (CE-MS) is widely used, however, direct analysis of low-abundance peptides such as histidine-containing peptides (His-peptides) in complex matrixes by CE-MS remains an analytical challenge. In the present study, an immobilised metal affinity solid-phase extraction containing Ni(II) coupled on-line to capillary electrophoresis-mass spectrometry (IMA-SPE-CE-MS) method has been developed to selectively enrich His-peptides from protein tryptic digests and enhance sensitivity. The method was optimised with α-casein and validated with other standard proteins (β-casein and κ-casein). Later, it was applied to an Escherichia Coli (E. Coli) whole cell lysate. IMA-SPE-CE-MS was very selective and allowed an enrichment factor up to 100-fold. The on-line enrichment and separation method coupled to MS detection is straightforward and advantageous over off-line pretreatment methods in terms of simplicity, cost-effectiveness and throughput. |
Note: | Versió postprint del document publicat a: https://doi.org/10.1016/j.microc.2020.105013 |
It is part of: | Microchemical Journal, 2020, vol. 267, p. 121881 |
URI: | http://hdl.handle.net/2445/162038 |
Related resource: | https://doi.org/10.1016/j.microc.2020.105013 |
ISSN: | 0026-265X |
Appears in Collections: | Articles publicats en revistes (Enginyeria Química i Química Analítica) |
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