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http://hdl.handle.net/2445/162210
Title: | Polymeric monolithic microcartridges with gold nanoparticles for the analysis of protein biomarkers by on-line solid-phase extraction capillary electrophoresis-mass spectrometry |
Author: | Pont Villanueva, Laura Marin, Gemma Vergara-Barberán, Maria Gagliardi, Leonardo G. Sanz Nebot, María Victoria Herrero-Martínez, J. M. Benavente Moreno, Fernando J. (Julián) |
Keywords: | Electroforesi capil·lar Or Nanopartícules Espectrometria de masses Capillary electrophoresis Gold Nanoparticles Mass spectrometry |
Issue Date: | 18-Apr-2020 |
Publisher: | Elsevier B.V. |
Abstract: | In this study, polymeric monoliths with gold nanoparticles (AuNP@monolith) were investigated as microcartridges for the analysis of protein biomarkers by on-line solid-phase extraction capillary electrophoresis-mass spectrometry (SPE-CE-MS). "Plug-and-play" microcartridges (7 mm) were prepared from a glycidyl methacrylate (GMA)-based monolithic capillary column (5 cm x 250 µm i.d.), which was modified with ammonia and subsequently functionalized with gold nanoparticles (AuNPs). The performance of these novel microcartridges was evaluated with human transthyretin (TTR), which is a protein related to different types of familial amyloidotic polyneuropathies (FAP). Protein retention depended on the isoelectric point of the protein (TTR pI~5.4) and elution was achieved with a basic phosphate solution. Under the optimized conditions, limits of detection (LODs) for TTR by AuNP@monolith-SPE-CE-MS were 50 times lower than by CE-MS (5 vs 250 mg L−1, with an ion trap (IT) mass spectrometer). The sensitivity enhancement was similar compared to SPE-CE-MS using immunoaffinity (IA) microcartridges with intact antibodies against TTR. Linearity, repeatability in migration times and peak areas, reusability, reproducibility and application to serum samples were also evaluated |
Note: | Versió postprint del document publicat a: https://doi.org/10.1016/j.chroma.2020.461097 |
It is part of: | Journal of Chromatography A, 2020, vol. 1622, p. 461097-461097 |
URI: | http://hdl.handle.net/2445/162210 |
Related resource: | https://doi.org/10.1016/j.chroma.2020.461097 |
ISSN: | 0021-9673 |
Appears in Collections: | Articles publicats en revistes (Enginyeria Química i Química Analítica) |
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