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Title: Liquid chromatography-tandem mass spectrometry analysis of eicosanoids and related compounds in cell models
Author: Martín Venegas, Raquel
Jáuregui Pallarés, Olga
Moreno Aznárez, Juan José
Keywords: Espectrometria de masses
Cromatografia de líquids
Cultiu cel·lular
Mass spectrometry
Liquid chromatography
Cell culture
Issue Date: 24-May-2014
Publisher: Elsevier B.V.
Abstract: Enzyme- and free radical-catalyzed oxidation of polyunsaturated fatty acids (PUFAs) produces the eicosanoids, docosanoids and octadecanoids. This large family of potent bioactive lipids is involved in many biochemical and signaling pathways which are implicated in physiological and pathophysiological processes and can be viable therapeutic targets. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) offers selectivity, sensitivity, robustness and high resolution and is able to analyze a large number of eicosanoids in biological samples in a short time. The present article reviews and discusses reported LC-MS/MS methods and the results obtained from their application in cell models. Reliable analytical outcomes are critically important for understanding physiological and pathophysiological cellular processes, such as inflammation, diseases with inflammatory components (e.g., cardiovascular disease, diabetes, metabolic syndrome), as well as cancer. Reported findings obtained by using the LC-MS/MS methodology in cell systems may have important predictive as well as nutritional and pharmacological implications. We conclude that the LC-MS/MS methodology is a versatile and reliable analytical tool for the simultaneous analysis of multiple PUFA-derived metabolites including the eicosanoids in cell culture samples at concentrations on the pM/nM threshold, i.e. at baseline and after stimulation.
Note: Versió postprint del document publicat a:
It is part of: Journal Of Chromatography B-Analytical Technologies In The Biomedical And Life Sciences, 2014, vol. 964, p. 41-49
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ISSN: 1570-0232
Appears in Collections:Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia)
Articles publicats en revistes (Centres Científics i Tecnològics de la Universitat de Barcelona (CCiTUB))
Articles publicats en revistes (Bioquímica i Fisiologia)

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