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Title: High-throughput analysis of lipid hydroperoxides in edible oils and fats using the fluorescent reagent diphenyl-1-pyrenylphosphine
Author: Santas Gutiérrez, Jonathan
Guzmán, Yeimmy J.
Guardiola Ibarz, Francesc
Rafecas Martínez, Magdalena
Bou Novensà, Ricard
Keywords: Olis i greixos comestibles
Colorants en els aliments
Compostos organofosforats
Reacció d'oxidació-reducció
Edible oils and fats
Food colorings
Organophosphorus compounds
Oxidation-reduction reaction
Issue Date: 1-Nov-2014
Publisher: Elsevier B.V.
Abstract: A fluorometric method for the determination of hydroperoxides (HP) in edible oils and fats using the reagent diphenyl-1-pyrenylphosphine (DPPP) was developed and validated. Two solvent media containing 100% butanol or a mixture of chloroform/methanol (2:1, v/v) can be used to solubilize lipid samples. Regardless of the solvent used to solubilize the sample, the DPPP method was precise, accurate, sensitive and easy to perform. The HP content of 43 oil and fat samples was determined and the results were compared with those obtained by means of the AOCS Official Method for the determination of peroxide value (PV) and the ferrous oxidation-xylenol orange (FOX) method. The proposed method not only correlates well with the PV and FOX methods, but also presents some advantages such as requiring low sample and solvent amounts and being suitable for high-throughput sample analysis.
Note: Versió postprint del document publicat a:
It is part of: Food Chemistry, 2014, vol. 162, p. 235-241
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ISSN: 0308-8146
Appears in Collections:Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia)

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