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http://hdl.handle.net/2445/164262| Title: | High-throughput analysis of lipid hydroperoxides in edible oils and fats using the fluorescent reagent diphenyl-1-pyrenylphosphine |
| Author: | Santas Gutiérrez, Jonathan Guzmán, Yeimmy J. Guardiola Ibarz, Francesc Rafecas Martínez, Magdalena Bou Novensà, Ricard |
| Keywords: | Olis i greixos comestibles Colorants en els aliments Peròxids Compostos organofosforats Reacció d'oxidació-reducció Fenols Solubilitat Dissolvents Edible oils and fats Food colorings Peroxides Organophosphorus compounds Oxidation-reduction reaction Phenols Solubility Solvents |
| Issue Date: | 1-Nov-2014 |
| Publisher: | Elsevier B.V. |
| Abstract: | A fluorometric method for the determination of hydroperoxides (HP) in edible oils and fats using the reagent diphenyl-1-pyrenylphosphine (DPPP) was developed and validated. Two solvent media containing 100% butanol or a mixture of chloroform/methanol (2:1, v/v) can be used to solubilize lipid samples. Regardless of the solvent used to solubilize the sample, the DPPP method was precise, accurate, sensitive and easy to perform. The HP content of 43 oil and fat samples was determined and the results were compared with those obtained by means of the AOCS Official Method for the determination of peroxide value (PV) and the ferrous oxidation-xylenol orange (FOX) method. The proposed method not only correlates well with the PV and FOX methods, but also presents some advantages such as requiring low sample and solvent amounts and being suitable for high-throughput sample analysis. |
| Note: | Versió postprint del document publicat a: https://doi.org/10.1016/j.foodchem.2014.04.059 |
| It is part of: | Food Chemistry, 2014, vol. 162, p. 235-241 |
| URI: | http://hdl.handle.net/2445/164262 |
| Related resource: | https://doi.org/10.1016/j.foodchem.2014.04.059 |
| ISSN: | 0308-8146 |
| Appears in Collections: | Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia) |
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| File | Description | Size | Format | |
|---|---|---|---|---|
| 642910.pdf | 1.51 MB | Adobe PDF | View/Open |
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