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Title: | Effect of E1(64-81) hepatitis G peptide on the in vitro interaction of HIV-1 Fusion Peptide with membrane models |
Author: | Sánchez Martín, Ma. Jesús Busquets i Viñas, Ma. Antonia Girona i Brumós, Victòria Haro, Isabel Alsina Esteller, Ma. Asunción Pujol Cubells, Montserrat |
Keywords: | Síntesi de pèptids Hepatitis G Virus GB C VIH (Virus) Peptide synthesis Hepatitis G GB virus C HIV (Viruses) |
Issue Date: | Sep-2011 |
Publisher: | Elsevier B.V. |
Abstract: | One way to gain information about the fusogenic potential of virus-derived synthetic peptides is to examine their interfacial properties and subsequently to study them in monolayers and bilayers. Here, we characterize the physicochemical surface properties of the peptide E1(64-81), whose sequence is AQLVGELGSLYGPLSVSA. This peptide is derived from the E1 structural protein of GBV-C/HGV which was previously shown to inhibit leakage of vesicular contents caused by the HIV-1 fusion peptide (HIV-1 FP). Mixed isotherms of E1(64-81) and HIV-1 FP were obtained and their Brewster angle microscopy (BAM) and atomic force microscopy (AFM) images showed that the peptide mixture forms a different structure that is not present in the pure peptide images. Studies with lipid monolayers (1,2-dimyristoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] (DMPG) and 1,2-dipalmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) (DPPG)) show that both peptides interact with all the lipids assayed but the effect that HIV-1 FP has on the monolayers is reduced in the presence of E1(64-81). Moreover, differential scanning calorimetry (DSC) experiments show the capacity of HIV-1 FP to modify the properties of the bilayer structure and the capacity of E1(64-81) to inhibit these modifications. Our results indicate that E1(64-81) interacts with HIV-1 FP to form a new structure, and that this may be the cause of the previously observed inhibition of the activity of HIV-1 FP by E1(64-81). |
Note: | Versió postprint del document publicat a: https://doi.org/10.1016/j.bbamem.2011.05.020 |
It is part of: | Biochimica et Biophysica Acta-Biomembranes, 2011, vol. 1808, num. 9, p. 2178-2188 |
URI: | http://hdl.handle.net/2445/164344 |
Related resource: | https://doi.org/10.1016/j.bbamem.2011.05.020 |
ISSN: | 0005-2736 |
Appears in Collections: | Articles publicats en revistes (Farmàcia, Tecnologia Farmacèutica i Fisicoquímica) Articles publicats en revistes (Institut de Nanociència i Nanotecnologia (IN2UB)) |
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595756.pdf | 2.09 MB | Adobe PDF | View/Open |
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