Please use this identifier to cite or link to this item:
Title: Water soluble, multifunctional antibody-porphyrin gold nanoparticles for targeted photodynamic therapy
Author: Penon Esteva, Oriol
Marín, María J.
Russell, David A.
Pérez García, M. Lluïsa (Maria Lluïsa)
Keywords: Porfirines
Issue Date: 2017
Publisher: Elsevier
Abstract: Photodynamic therapy (PDT) is a treatment of cancer by which tumour cells are destroyed using reactive oxygen species produced by photosensitisers following activation with visible or near infrared light. Successful PDT depends on the solubility and the targeting ability of the photosensitisers. In this work, the synthesis of a porphyrin-based water soluble nanoparticle conjugate containing a targeting agent that recognises the erbB2 receptor overexpressed on the surface of particular cancer cells is reported. The nanoparticle conjugates were synthesised following two different protocols, viz. a biphasic and a monophasic method, with the aim to determine which method yielded the optimal nanosystem for potential PDT applications. The nanoparticles were characterised using UV-Vis absorption and fluorescence spectroscopies together with transmission electron microscopy and zeta potential measurements; and their ability to produce singlet oxygen following irradiation was investigated following the decay in absorption of a singlet oxygen probe. The nanoparticles synthesised using the monophasic method were shown to produce the highest amount of singlet oxygen and were further functionalised with anti-erbB2 antibody to target the erbB2 receptors expressed on the surface of SK-BR-3 human breast cancer cells. The water soluble, antibody-porphyrin nanoparticle conjugates were shown to elicit targeted PDT of the breast cancer cells. Keywords: Photodynamic therapy, Multifunctional nanoparticles, Porphyrins, Gold nanoparticles (AuNPs), Water soluble nanoconjugates, Targeting antibodies, Singlet oxygen production, In vitro phototoxicity, Human breast cancer cells
Note: Versió postprint del document publicat a:
It is part of: Journal of Colloid and Interface Science, 2017, vol. 496, p. 100-110
Related resource:
ISSN: 0021-9797
Appears in Collections:Articles publicats en revistes (Farmacologia, Toxicologia i Química Terapèutica)

Files in This Item:
File Description SizeFormat 
670265.pdf1.44 MBAdobe PDFView/Open

This item is licensed under a Creative Commons License Creative Commons