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http://hdl.handle.net/2445/171714
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DC Field | Value | Language |
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dc.contributor.author | El Mouali Benomar, Youssef | - |
dc.contributor.author | Esteva-Martínez, Guillem | - |
dc.contributor.author | García-Pedemonte, David | - |
dc.contributor.author | Balsalobre Parra, Carlos | - |
dc.date.accessioned | 2020-11-03T09:36:07Z | - |
dc.date.available | 2020-11-03T09:36:07Z | - |
dc.date.issued | 2020-10-14 | - |
dc.identifier.issn | 1664-302X | - |
dc.identifier.uri | http://hdl.handle.net/2445/171714 | - |
dc.description.abstract | Post-transcriptional regulation mediated by regulatory small RNAs (sRNAs) has risen as a key player in fine-tuning gene expression in response to environmental stimuli. Here, we show that, in Salmonella enterica, the central metabolic regulator CRP-cAMP differentially regulates the sRNAs CsrB and CsrC in a growth phase-dependent manner. While CsrB expression remains unchanged during growth, CsrC displays a growth phase-dependent expression profile, being weakly expressed at the logarithmic growth phase and induced upon entry into stationary phase. We show that CRP-cAMP contributes to the expression pattern of CsrC by repressing its expression during the logarithmic growth phase. The CRP-cAMP mediated repression of CsrC is independent of SirA, a known transcriptional CsrB/CsrC activator. We further show that the sRNA Spot 42, which is derepressed in a Δcrp strain, upregulates CsrC during logarithmic growth. We propose a model where the growth-dependent regulation of CsrC is sustained by the CRP-cAMP-mediated repression of Spot 42. Together, our data point toward a differential regulation of the sRNAs CsrB and CsrC in response to environmental stimuli, leading to fine-tuning of gene expression via the sequestration of the RNA-binding protein CsrA. | - |
dc.format.extent | 9 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Frontiers Media | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.3389/fmicb.2020.570536 | - |
dc.relation.ispartof | Frontiers in Microbiology, 2020, vol. 11, p. 570536 | - |
dc.relation.uri | https://doi.org/10.3389/fmicb.2020.570536 | - |
dc.rights | cc-by (c) El Mouali, Youssef et al., 2020 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Genètica, Microbiologia i Estadística) | - |
dc.subject.classification | Salmonel·la | - |
dc.subject.classification | Expressió gènica | - |
dc.subject.other | Salmonella | - |
dc.subject.other | Gene expression | - |
dc.title | Differential regulation of CsrC and CsrB by CRP-cAMP in Salmonella enterica | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 703926 | - |
dc.date.updated | 2020-11-03T09:36:08Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 19521501 | - |
dc.identifier.pmid | 33162952 | - |
Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) |
Files in This Item:
File | Description | Size | Format | |
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703926.pdf | 1.63 MB | Adobe PDF | View/Open |
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