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Title: Somatostatin, an in vivo binder to Aβ oligomers, Binds to βPFOAβ(1−42) Tetramers
Author: Puig Gomà-Camps, Eduard
Tolchard, James
Riera i Escalé, Antoni
Carulla Casanovas, Natàlia
Keywords: Malaltia d'Alzheimer
Alzheimer's disease
Issue Date: 11-Sep-2020
Publisher: American Chemical Society
Abstract: Somatostatin (SST14) is strongly related to Alzheimer's disease (AD), as its levels decline during aging, it regulates the proteolytic degradation of the amyloid beta peptide (Aβ), and it binds to Aβ oligomers in vivo. Recently, the 3D structure of a membrane-associated β-sheet pore-forming tetramer (βPFOAβ(1−42) tetramer) has been reported. Here, we show that SST14 binds selectively to the βPFOAβ(1−42) tetramer with a KD value of ∼40 μM without binding to monomeric Aβ(1−42). Specific NMR chemical shift perturbations, observed during titration of SST14, define a binding site in the βPFOAβ(1−42) tetramer and are in agreement with a 2:1 stoichiometry determined by both native mass spectroscopy and isothermal titration calorimetry. These results enabled us to perform driven docking and model the binding mode for the interaction. The present study provides additional evidence on the relation between SST14 and the amyloid cascade and positions the βPFOAβ(1−42) tetramer as a relevant aggregation form of Aβ and as a potential target for AD.
Note: Versió postprint del document publicat a:
It is part of: Acs Chemical Neuroscience, 2020, vol. 11, num. 20, p. 3358-3365
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ISSN: 1948-7193
Appears in Collections:Articles publicats en revistes (Química Inorgànica i Orgànica)

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