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Title: | Sipa1l1 is an early biomarker of liver fibrosis in CCl4-treated rats |
Author: | Fernández Varo, Guillermo Jiménez Povedano, Wladimiro Marfà Bruix, Santiago Morales Ruiz, Manuel Oró Bozzini, Denise Ribera Sabaté, Jordi |
Keywords: | Marcadors bioquímics Proteòmica Malalties del fetge Biochemical markers Proteomics Liver diseases |
Issue Date: | 15-Jun-2016 |
Abstract: | At present, several procedures are used for staging liver fibrosis. However, these methods may involve clinical complications and/or present diagnostic uncertainty mainly in the early stages of the disease. Thus, this study was designed to unveil new non-invasive biomarkers of liver fibrosis in an in vivo model of fibrosis/cirrhosis induction by CCl4 inhalation by using a label-free quantitative LC-MS/MS approach. We analyzed 94 serum samples from adult Wistar rats with different degrees of liver fibrosis and 36 control rats. Firstly, serum samples from 18 CCl4-treated rats were clustered into three different groups according to the severity of hepatic and the serum proteome was characterized by label-free LC-MS/MS. Furthermore, three different pooled serum samples obtained from 16 control Wistar rats were also analyzed. Based on the proteomic data obtained, we performed a multivariate analysis which displayed three main cell signaling pathways altered in fibrosis. In cirrhosis, more biological imbalances were detected as well as multi-organ alterations. In addition, hemopexin and signal-induced proliferation-associated 1 like 1 (SIPA1L1) were selected as potential serum markers of liver fibrogenesis among all the analyzed proteins. The results were validated by ELISA in an independent group of 76 fibrotic/cirrhotic rats and 20 controls which confirmed SIPA1L1 as a potential non-invasive biomarker of liver fibrosis. In particular, SIPA1L1 showed a clear diminution in serum samples from fibrotic/cirrhotic rats and a great accuracy at identifying early fibrotic stages. In conclusion, the proteomic analysis of serum samples from CCl4-treated rats has enabled the identification of SIPA1L1 as a non-invasive marker of early liver fibrosis. © 2016. Published by The Company of Biologists Ltd. |
Note: | Reproducció del document publicat a: https://doi.org/10.1242/bio.018887 |
It is part of: | Biology Open, 2016, vol. 5, p. 858-865 |
URI: | http://hdl.handle.net/2445/173186 |
Related resource: | https://doi.org/10.1242/bio.018887 |
Appears in Collections: | Articles publicats en revistes (Biomedicina) Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) |
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