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DC Field | Value | Language |
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dc.contributor.author | Gibert, Marta | - |
dc.contributor.author | Paytubi Casabona, Sònia | - |
dc.contributor.author | Madrid Xufré, Cristina | - |
dc.contributor.author | Balsalobre Parra, Carlos | - |
dc.date.accessioned | 2021-01-29T12:05:12Z | - |
dc.date.available | 2021-01-29T12:05:12Z | - |
dc.date.issued | 2020-07-10 | - |
dc.identifier.issn | 2296-889X | - |
dc.identifier.uri | http://hdl.handle.net/2445/173522 | - |
dc.description.abstract | Conjugation of R27 plasmid is thermoregulated, being promoted at 25°C and repressed at 37°C. Previous studies identified plasmid-encoded regulators, HtdA, TrhR and TrhY, that control expression of conjugation-related genes (tra). Moreover, the nucleoid-associated protein H-NS represses conjugation at non-permissive temperature. A transcriptomic approach has been used to characterize the effect of temperature on the expression of the 205 R27 genes. Many of the 35 tra genes, directly involved in plasmid-conjugation, were upregulated at 25°C. However, the majority of the non-tra R27 genes many of them with unknown function were more actively expressed at 37°C. The role of HtdA, a regulator that causes repression of the R27 conjugation by counteracting TrhR/TrhY mediated activation of tra genes, has been investigated. Most of the R27 genes are severely derepressed at 25°C in an htdA mutant, suggesting that HtdA is involved also in the repression of R27 genes other than the tra genes. Interestingly, the effect of htdA mutation was abolished at non-permissive temperature, indicating that the HtdA-TrhR/TrhY regulatory circuit mediates the environmental regulation of R27 gene expression. The role of H-NS in the proposed model is discussed. | - |
dc.format.extent | 10 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Frontiers Media | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.3389/fmolb.2020.00124 | - |
dc.relation.ispartof | Frontiers In Molecular Biosciences, 2020, num. 7 | - |
dc.relation.uri | https://doi.org/10.3389/fmolb.2020.00124 | - |
dc.rights | cc-by (c) Gibert, Marta et al., 2020 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Genètica, Microbiologia i Estadística) | - |
dc.subject.classification | Plasmidis | - |
dc.subject.classification | Expressió gènica | - |
dc.subject.other | Plasmids | - |
dc.subject.other | Gene expression | - |
dc.title | Temperature Dependent Control of the R27 Conjugative Plasmid Genes | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 702179 | - |
dc.date.updated | 2021-01-29T12:05:12Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 32754612 | - |
Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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702179.pdf | 2.88 MB | Adobe PDF | View/Open |
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