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Title: Inactivation of hepatitis A virus and human norovirus in clams subjected to heat treatment
Author: Fuentes Pardo, Cristina
Pérez-Rodríguez, Francisco Javier
Sabrià Sunyé, Aurora
Beguiristain Celayeta, Nerea
Pintó Solé, Rosa María
Guix Arnau, Susana
Bosch, Albert
Keywords: Hepatitis A
Hepatitis A
Issue Date: 12-Jan-2021
Publisher: Frontiers Media
Abstract: Bivalve mollusk contamination by enteric viruses, especially human noroviruses (HuNoV) and hepatitis A virus (HAV), is a problem with health and economic implications. The aim of the study was the evaluation of the effect of heat treatment in clams (Tawera gayi) experimentally contaminated with HuNoV using a PMA-viability RTqPCR assay to minimize measurement of non-infectious viruses, and used HAV as a model to estimate infectivity loss. Spiked clams were immersed in water at 90°C to ensure that internal meat temperature was maintained above 90°C for at least 5 min. The treatment resulted in >3.89 ± 0.24 log10 TCID50/g reduction of infectious HAV, confirming inactivation. For HuNoV, RTqPCR assays showed log10 reductions of 2.96 ± 0.79 and 2.56 ± 0.56, for GI and GII, respectively, and the use of PMA resulted in an additional log10 reduction for GII, providing a better correlation with risk reduction. In the absence of a cell culture system which could be used to determine HuNoV infectivity reduction, a performance criteria based on PMA-RTqPCR log reduction could be used to evaluate food product safety. According to data from this study, heat treatments of clams which cause reductions >3.5 log10 for GII as measured by PMA-RTqPCR assay may be regarded as an acceptable inactivation treatment, and could be set as a performance criterion to test the effectiveness of other time-temperature inactivation processes.
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It is part of: Frontiers in Microbiology, 2021, vol. 11, p. 578328
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ISSN: 1664-302X
Appears in Collections:Publicacions de projectes de recerca finançats per la UE
Articles publicats en revistes (Genètica, Microbiologia i Estadística)

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