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Title: Development and application of a one-step low cost procedure to concentrate viruses from seawater samples
Author: Calgua de León, Byron Thomas
Mengewein, A.
Grünert, Andreas
Bofill Mas, Silvia
Clemente Casares, Pilar
Hundesa Gonfa, Ayalkibet
Wyn-Jones, A. Peter
López-Pila, Juan
Gironès Llop, Rosina
Keywords: Adenovirus
Aigua de mar
Issue Date: 2008
Publisher: Elsevier B.V.
Abstract: A novel and simple procedure for concentrating adenoviruses from seawater samples is described. The technique entails the adsorption of viruses to pre-flocculated skimmed milk proteins, allowing the flocs to sediment by gravity, and dissolving the separated sediment in phosphate buffer. Concentrated virus may be detected by PCR techniques following nucleic acid extraction. The method requires no specialized equipment other than that usually available in routine public health laboratories, and due to its straightforwardness it allows the processing of a larger number of water samples simultaneously. The usefulness of the method was demonstrated in concentration of virus in multiple seawater samples during a survey of adenoviruses in coastal waters.
Note: Versió postprint del document publicat a
It is part of: Journal of Virological Methods, 2008, volumen 153, p. 79-83
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ISSN: 0166-0934
Appears in Collections:Articles publicats en revistes (Genètica, Microbiologia i Estadística)
Publicacions de projectes de recerca finançats per la UE

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