Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/175743
Title: | Diagnostic usefulness of immunohistochemical evaluation of CD1a antigen and polyclonal anti-leishmania antibodies in cutaneous leishmaniasis |
Author: | Lopez-Trujillo, Emilio González Farré, Mónica Pujol, Ramon M. Bellosillo, Beatriz Fisa Saladrigas, Roser Riera Lizandra, Ma. Cristina Alcover Amengual, Maria Magdalena Barranco, Carlos Martin-Ezquerra, Gemma |
Keywords: | Leishmània Leishmaniosi Antígens Leishmania Leishmaniasis Antigens |
Issue Date: | 4-Mar-2021 |
Publisher: | Sercrisma International |
Abstract: | Background: Different immunohistochemical markers to detect amastigotes in cutaneous leishmaniasis have been proposed with variable diagnostic usefulness. Objectives: To evaluate the diagnostic usefulness of immunohistochemical amastigotes identification by specific polyclonal anti-Leishmania antibodies and CD1a expression (clone EP3622) in a series of PCR confirmed cutaneous leishmaniasis. Materials and methods: Thirty-three skin samples corresponding to PCR confirmed cutaneous leishmaniasis were included in the study. All samples were stained with Hematoxylin-eosin and Giemsa. Moreover, immunohistochemical studies with anti-CD1a and anti-Leishmania antibodies were performed. The patients clinical features and the observed histopathological features were also recorded. Results: From the selected 33 biopsies, Leishmania spp. amastigotes were detected in 48.4% of cases with conventional Hematoxylin-eosin stain and in 57.5% of cases by Giemsa staining. In 31/33 cases, anti-CD1a allowed us to identify parasitic structures, and in 33/33 cases amastigotes were detected with anti-Leishmania antibodies. Concordance between both techniques, anti-CD1a and anti-Leishmania, was 94% [CI 95%: (79,8%-99,3%)] ; p value <0.05. The sensitivity of anti-CD1a in comparison with the PCR was 94%, with a positive predictive value of 100%. Two cases of low parasitic index were negative for CD1a immunostaining. In cases with high parasitic index, anti-CD1a stained amastigotes in superficial and deep dermis. Only a few cases were originally diagnosed with the available histological techniques, needing PCR for Leishmania spp. Conclusions: Anti-CD1a antibody seems to be a useful technique to identify amastigotes when PCR and anti-Leishmania antibodies are not available. The sensitivity to detect amastigotes is increased when the CD1a immunostaining is added to the classical Haematoxylin - eosin and Giemsa staining. |
Note: | Reproducció del document publicat a: https://doi.org/10.14670/HH-18-324 |
It is part of: | Histology and Histopathology, 2021 |
URI: | http://hdl.handle.net/2445/175743 |
Related resource: | https://doi.org/10.14670/HH-18-324 |
ISSN: | 0213-3911 |
Appears in Collections: | Articles publicats en revistes (Biologia, Sanitat i Medi Ambient) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
707955.pdf | 1.14 MB | Adobe PDF | View/Open Request a copy |
Document embargat fins el 4-3-2026
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.