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Title: Quantification of human adenoviruses in European recreational waters
Author: Bofill Mas, Silvia
Calgua de León, Byron Thomas
Clemente Casares, Pilar
La Rosa, Giuseppina
Iaconelli, Marcello
Muscillo, Michele
Rutjes, Saskia
Roda Husman, Ana Maria de
Grünert, Andreas
Graver, Ingeburg
Verani, Marco
Carducci, Annalaura
Calvo, Miquel
Wyn-Jones, A. Peter
Gironès Llop, Rosina
Keywords: Adenovirus
Aigua de mar
Aigua dolça
Issue Date: 2010
Publisher: Springer
Abstract: The presence of human adenoviruses in recreational water might cause disease in the population upon exposure. Human adenoviruses detected by PCR could also serve as indicators of the virological water quality. In order to assess the applicability of human adenoviruses to the evaluation of the faecal contamination in European bathing waters, a real-time quantitative PCR assay was developed for the quantification of human adenoviruses in 132 samples collected from 24 different recreational marine and freshwater sites in nine European countries. Selected samples presenting positive nested-PCR results for human adenoviruses were analyzed using quantitative PCR and 80 samples from a total of 132 produced quantitative results with mean values of 3.2x102 10 per 100 ml of water, human adenovirus 41 being the most prevalent serotype. Human adenoviruses were quantified in samples from all 15 surveillance laboratories. Statistical analysis showed no homogeneous linear relation between human adenoviruses and E. coli, intestinal enterococci or somatic coliphages concentrations in the tested samples when considering all the data together. Significant correlations between human adenoviruses and at least one of the other indicators were observed only when data from individual Laboratories were considered. The quantification of human adenoviruses may provide complementary information in relation to the use of bacterial standards in the control of water quality in bathing water.
Note: Versió postprint del document publicat a :
It is part of: Food and Environmental Virology, 2010, vol. 2, p. 101-109
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ISSN: 1867-0334
Appears in Collections:Articles publicats en revistes (Genètica, Microbiologia i Estadística)
Publicacions de projectes de recerca finançats per la UE

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