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DC Field | Value | Language |
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dc.contributor.author | Arato, Ivo | - |
dc.contributor.author | Grande, Giuseppe | - |
dc.contributor.author | Barrachina, Ferran | - |
dc.contributor.author | Bellucci, Catia | - |
dc.contributor.author | Lilli, Cinzia | - |
dc.contributor.author | Jodar Bifet, Meritxell | - |
dc.contributor.author | Aglietti, Maria Chiara | - |
dc.contributor.author | Mancini, Francesca | - |
dc.contributor.author | Vincenzoni, Federica | - |
dc.contributor.author | Pontecorvi, Alfredo | - |
dc.contributor.author | Calafiore, Riccardo | - |
dc.contributor.author | Oliva Virgili, Rafael | - |
dc.contributor.author | Luca, Giovanni | - |
dc.contributor.author | Mancuso, Francesca | - |
dc.contributor.author | Milardi, Domenico | - |
dc.date.accessioned | 2021-04-22T09:31:34Z | - |
dc.date.available | 2021-04-22T09:31:34Z | - |
dc.date.issued | 2020-06-18 | - |
dc.identifier.issn | 1664-2392 | - |
dc.identifier.uri | http://hdl.handle.net/2445/176569 | - |
dc.description.abstract | Follicle-stimulating hormone (FSH), a major regulator of spermatogenesis, has a crucial function in the development and function of the testis and it is extensively given as a fertility treatment to stimulate spermatogenesis. We analyzed the effects of different FSH preparations (α-follitropin, β-follitropin, and urofollitropin) in combination with testosterone on porcine pre-pubertal Sertoli cells. To study the effect of the different FSH treatments in the Sertoli cell function we performed Real Time PCR analysis of AMH, inhibin B, and FSH-r, an ELISA assay for AMH and inhibin B, and a high-throughput comparative proteomic analysis. We verified that all three preparations induced a reduction of AMH in terms of mRNA and secreted proteins, and an increase of inhibin B in terms of mRNA in all the FSH formulations, while solely α-follitropin produced an increase of secreted inhibin B in the culture medium. Comparative proteomic analysis of the three FSH preparations identified 46 proteins, 11 up-regulated and 2 down-regulated. Surprisingly, the combination of testosterone with β-follitropin specifically induced an up-regulation of eight specific secreted proteins. Our study, showing that the three different FSH preparations induce different effects, could offer the opportunity to shed light inside new applications to a personalized reproductive medicine. | - |
dc.format.extent | 10 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Frontiers Media | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.3389/fendo.2020.00401 | - |
dc.relation.ispartof | Frontiers In Endocrinology, 2020, vol. 11, p. 401 | - |
dc.relation.uri | https://doi.org/10.3389/fendo.2020.00401 | - |
dc.rights | cc-by (c) Arato, Ivo et al., 2020 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Biomedicina) | - |
dc.subject.classification | Cèl·lules de Sertoli | - |
dc.subject.classification | Espermatogènesi | - |
dc.subject.other | Sertoli cells | - |
dc.subject.other | Spermatogenesis | - |
dc.title | 'In vitro' Effect of Different Follicle¿Stimulating Hormone Preparations on Sertoli Cells: Toward a Personalized Treatment for Male Infertility | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 709772 | - |
dc.date.updated | 2021-04-22T09:31:34Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 32625170 | - |
Appears in Collections: | Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) Articles publicats en revistes (Biomedicina) |
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709772.pdf | 606.27 kB | Adobe PDF | View/Open |
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