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Title: | 'In vitro' Effect of Different Follicle¿Stimulating Hormone Preparations on Sertoli Cells: Toward a Personalized Treatment for Male Infertility |
Author: | Arato, Ivo Grande, Giuseppe Barrachina, Ferran Bellucci, Catia Lilli, Cinzia Jodar Bifet, Meritxell Aglietti, Maria Chiara Mancini, Francesca Vincenzoni, Federica Pontecorvi, Alfredo Calafiore, Riccardo Oliva Virgili, Rafael Luca, Giovanni Mancuso, Francesca Milardi, Domenico |
Keywords: | Cèl·lules de Sertoli Espermatogènesi Sertoli cells Spermatogenesis |
Issue Date: | 18-Jun-2020 |
Publisher: | Frontiers Media |
Abstract: | Follicle-stimulating hormone (FSH), a major regulator of spermatogenesis, has a crucial function in the development and function of the testis and it is extensively given as a fertility treatment to stimulate spermatogenesis. We analyzed the effects of different FSH preparations (α-follitropin, β-follitropin, and urofollitropin) in combination with testosterone on porcine pre-pubertal Sertoli cells. To study the effect of the different FSH treatments in the Sertoli cell function we performed Real Time PCR analysis of AMH, inhibin B, and FSH-r, an ELISA assay for AMH and inhibin B, and a high-throughput comparative proteomic analysis. We verified that all three preparations induced a reduction of AMH in terms of mRNA and secreted proteins, and an increase of inhibin B in terms of mRNA in all the FSH formulations, while solely α-follitropin produced an increase of secreted inhibin B in the culture medium. Comparative proteomic analysis of the three FSH preparations identified 46 proteins, 11 up-regulated and 2 down-regulated. Surprisingly, the combination of testosterone with β-follitropin specifically induced an up-regulation of eight specific secreted proteins. Our study, showing that the three different FSH preparations induce different effects, could offer the opportunity to shed light inside new applications to a personalized reproductive medicine. |
Note: | Reproducció del document publicat a: https://doi.org/10.3389/fendo.2020.00401 |
It is part of: | Frontiers In Endocrinology, 2020, vol. 11, p. 401 |
URI: | http://hdl.handle.net/2445/176569 |
Related resource: | https://doi.org/10.3389/fendo.2020.00401 |
ISSN: | 1664-2392 |
Appears in Collections: | Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) Articles publicats en revistes (Biomedicina) |
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