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http://hdl.handle.net/2445/177123
Title: | Identification of a membrane protein, LAT-2, that co-expresses with 4F2 heavy chain, an L-type amino acid transport activity with broad specificity for small and large zwitterionic amino acids |
Author: | Pineda Riu, Marta Fernández, Esperanza Torrents Arenales, David Estévez Povedano, Raúl López Iglesias, Carmen Camps Camprubí, Marta Lloberas Cavero, Jorge Zorzano Olarte, Antonio Palacín Prieto, Manuel |
Keywords: | Antígens CD Proteïnes portadores Proteïnes de membrana CD antigens Carrier proteins Membrane proteins |
Issue Date: | 9-Jul-1999 |
Publisher: | American Society for Biochemistry and Molecular Biology |
Abstract: | We have identified a new human cDNA, L-amino acid transporter-2 (LAT-2), that induces a system L transport activity with 4F2hc (the heavy chain of the surface antigen 4F2, also named CD98) in oocytes. Human LAT-2 is the fourth member of the family of amino acid transporters that are subunits of 4F2hc. The amino acid transport activity induced by the co-expression of 4F2hc and LAT-2 was sodium-independent and showed broad specificity for small and large zwitterionic amino acids, as well as bulky analogs (e.g. BCH (2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid)). This transport activity was highly trans-stimulated, suggesting an exchanger mechanism of transport. Expression of tagged N-myc-LAT-2 alone in oocytes did not induce amino acid transport, and the protein had an intracellular location. Co-expression of N-myc-LAT-2 and 4F2hc gave amino acid transport induction and expression of N-myc-LAT-2 at the plasma membrane of the oocytes. These data suggest that LAT-2 is an additional member of the family of 4F2 light chain subunits, which associates with 4F2hc to express a system L transport activity with broad specificity for zwitterionic amino acids. Human LAT-2 mRNA is expressed in kidney >>> placenta >> brain, liver > spleen, skeletal muscle, heart, small intestine, and lung. Human LAT-2 gene localizes at chromosome 14q11.2-13 (13 cR or approximately 286 kb from marker D14S1349). The high expression of LAT-2 mRNA in epithelial cells of proximal tubules, the basolateral location of 4F2hc in these cells, and the amino acid transport activity of LAT-2 suggest that this transporter contributes to the renal reabsorption of neutral amino acids in the basolateral domain of epithelial proximal tubule cells. |
Note: | Reproducció del document publicat a: https://doi.org/10.1074/jbc.274.28.19738 |
It is part of: | Journal of Biological Chemistry, 1999, vol. 274, num. 28, p. 19738-19744 |
URI: | http://hdl.handle.net/2445/177123 |
Related resource: | https://doi.org/10.1074/jbc.274.28.19738 |
ISSN: | 0021-9258 |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Biomedicina Molecular) Articles publicats en revistes (Ciències Fisiològiques) Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia) |
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