Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/177472
Title: Oleacein Intestinal Permeation and Metabolism in Rats Using an In Situ Perfusion Technique
Author: López-Yerena, Anallely
Pérez Bosch, Maria
Vallverdú i Queralt, Anna
Miliarakis, Eleftherios
Lamuela Raventós, Rosa Ma.
Escribano Ferrer, Elvira
Keywords: Metabolisme
Permeabilitat
Intestins
Oli d'oliva
Metabolism
Permeability
Intestines
Olive oil
Issue Date: 12-May-2021
Publisher: MDPI
Abstract: Oleacein (OLEA) is one of the most important phenolic compounds in extra virgin olive oil in terms of concentration and health-promoting properties, yet there are insufficient data on its absorption and metabolism. Several non-human models have been developed to assess the intestinal permeability of drugs, among them, single-pass intestinal perfusion (SPIP), which is commonly used to investigate the trans-membrane transport of drugs in situ. In this study, the SPIP model and simultaneous luminal blood sampling were used to study the absorption and metabolism of OLEA in rats. Samples of intestinal fluid and mesenteric blood were taken at different times and the ileum segment was excised at the end of the experiment for analysis by LC-ESI-LTQ-Orbitrap-MS. OLEA was mostly metabolized by phase I reactions, undergoing hydrolysis and oxidation, and metabolite levels were much higher in the plasma than in the lumen. The large number of metabolites identified and their relatively high abundance indicates an important intestinal first-pass effect during absorption. According to the results, OLEA is well absorbed in the intestine, with an intestinal permeability similar to that of the highly permeable model compound naproxen. No significant differences were found in the percentage of absorbed OLEA and naproxen (48.98 ± 12.27% and 43.96 ± 7.58%, respectively).
Note: Reproducció del document publicat a: https://doi.org/10.3390/pharmaceutics13050719
It is part of: Pharmaceutics, 2021, vol. 13, num. 5, p. 719
URI: http://hdl.handle.net/2445/177472
Related resource: https://doi.org/10.3390/pharmaceutics13050719
ISSN: 1999-4923
Appears in Collections:Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia)

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