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DC Field | Value | Language |
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dc.contributor.author | Soler Prat, Concepció | - |
dc.contributor.author | Valdés, Raquel | - |
dc.contributor.author | García-Manteiga, José | - |
dc.contributor.author | Xaus Pey, Jordi | - |
dc.contributor.author | Comalada Vila, Mònica | - |
dc.contributor.author | Casado, Javier (Casado Merediz) | - |
dc.contributor.author | Modolell, Manuel | - |
dc.contributor.author | MacLeod, Carol | - |
dc.contributor.author | Nicholson, Benjamin | - |
dc.contributor.author | Felipe Campo, Antonio | - |
dc.contributor.author | Celada Cotarelo, Antonio | - |
dc.contributor.author | Pastor Anglada, Marçal | - |
dc.date.accessioned | 2021-05-20T13:59:52Z | - |
dc.date.available | 2021-05-20T13:59:52Z | - |
dc.date.issued | 2001-08-10 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | http://hdl.handle.net/2445/177485 | - |
dc.description.abstract | In murine bone marrow macrophages, lipopolysaccharide (LPS) induces apoptosis through the autocrine production of tumor necrosis factor-alpha (TNF-alpha), as demonstrated by the fact that macrophages from TNF-alpha receptor I knock-out mice did not undergo early apoptosis. In these conditions LPS up-regulated the two concentrative high affinity nucleoside transporters here shown to be expressed in murine bone marrow macrophages, concentrative nucleoside transporter (CNT) 1 and 2, in a rapid manner that is nevertheless consistent with the de novo synthesis of carrier proteins. This effect was not dependent on the presence of macrophage colony-stimulating factor, although LPS blocked the macrophage colony-stimulating factor-mediated up-regulation of the equilibrative nucleoside transport system es. TNF-alpha mimicked the regulatory response of nucleoside transporters triggered by LPS, but macrophages isolated from TNF-alpha receptor I knock-out mice similarly up-regulated nucleoside transport after LPS treatment. Although NO is produced by macrophages after LPS treatment, NO is not involved in these regulatory responses because LPS up-regulated CNT1 and CNT2 transport activity and expression in macrophages from inducible nitric oxide synthase and cationic amino acid transporter (CAT) 2 knock-out mice, both of which lack inducible nitric oxide synthesis. These data indicate that the early proapoptotic responses of macrophages, involving the up-regulation of CNT transporters, follow redundant regulatory pathways in which TNF-alpha-dependent- and -independent mechanisms are involved. These observations also support a role for CNT transporters in determining extracellular nucleoside availability and modulating macrophage apoptosis. | - |
dc.format.extent | 7 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | American Society for Biochemistry and Molecular Biology | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1074/jbc.M101807200 | - |
dc.relation.ispartof | Journal of Biological Chemistry, 2001, vol. 276, num. 32, p. 30043-30049 | - |
dc.relation.uri | https://doi.org/10.1074/jbc.M101807200 | - |
dc.rights | (c) American Society for Biochemistry and Molecular Biology, 2001 | - |
dc.source | Articles publicats en revistes (Patologia i Terapèutica Experimental) | - |
dc.subject.classification | Apoptosi | - |
dc.subject.classification | Proteïnes portadores | - |
dc.subject.classification | Macròfags | - |
dc.subject.classification | Necrosi | - |
dc.subject.other | Apoptosis | - |
dc.subject.other | Carrier proteins | - |
dc.subject.other | Macrophages | - |
dc.subject.other | Necrosis | - |
dc.title | Lipopolysaccharide-induced apoptosis of macrophages determines the up-regulation of concentrative nucleoside transporters Cnt1 and Cnt2 through tumor necrosis factor-alpha-dependent and -independent mechanisms | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 178527 | - |
dc.date.updated | 2021-05-20T13:59:52Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 11346649 | - |
Appears in Collections: | Articles publicats en revistes (Patologia i Terapèutica Experimental) Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia) Articles publicats en revistes (Bioquímica i Biomedicina Molecular) |
Files in This Item:
File | Description | Size | Format | |
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178527.pdf | 544.2 kB | Adobe PDF | View/Open |
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