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Title: | Lipopolysaccharide-induced apoptosis of macrophages determines the up-regulation of concentrative nucleoside transporters Cnt1 and Cnt2 through tumor necrosis factor-alpha-dependent and -independent mechanisms |
Author: | Soler Prat, Concepció Valdés, Raquel García-Manteiga, José Xaus Pey, Jordi Comalada Vila, Mònica Casado, Javier (Casado Merediz) Modolell, Manuel MacLeod, Carol Nicholson, Benjamin Felipe Campo, Antonio Celada Cotarelo, Antonio Pastor Anglada, Marçal |
Keywords: | Apoptosi Proteïnes portadores Macròfags Necrosi Apoptosis Carrier proteins Macrophages Necrosis |
Issue Date: | 10-Aug-2001 |
Publisher: | American Society for Biochemistry and Molecular Biology |
Abstract: | In murine bone marrow macrophages, lipopolysaccharide (LPS) induces apoptosis through the autocrine production of tumor necrosis factor-alpha (TNF-alpha), as demonstrated by the fact that macrophages from TNF-alpha receptor I knock-out mice did not undergo early apoptosis. In these conditions LPS up-regulated the two concentrative high affinity nucleoside transporters here shown to be expressed in murine bone marrow macrophages, concentrative nucleoside transporter (CNT) 1 and 2, in a rapid manner that is nevertheless consistent with the de novo synthesis of carrier proteins. This effect was not dependent on the presence of macrophage colony-stimulating factor, although LPS blocked the macrophage colony-stimulating factor-mediated up-regulation of the equilibrative nucleoside transport system es. TNF-alpha mimicked the regulatory response of nucleoside transporters triggered by LPS, but macrophages isolated from TNF-alpha receptor I knock-out mice similarly up-regulated nucleoside transport after LPS treatment. Although NO is produced by macrophages after LPS treatment, NO is not involved in these regulatory responses because LPS up-regulated CNT1 and CNT2 transport activity and expression in macrophages from inducible nitric oxide synthase and cationic amino acid transporter (CAT) 2 knock-out mice, both of which lack inducible nitric oxide synthesis. These data indicate that the early proapoptotic responses of macrophages, involving the up-regulation of CNT transporters, follow redundant regulatory pathways in which TNF-alpha-dependent- and -independent mechanisms are involved. These observations also support a role for CNT transporters in determining extracellular nucleoside availability and modulating macrophage apoptosis. |
Note: | Reproducció del document publicat a: https://doi.org/10.1074/jbc.M101807200 |
It is part of: | Journal of Biological Chemistry, 2001, vol. 276, num. 32, p. 30043-30049 |
URI: | http://hdl.handle.net/2445/177485 |
Related resource: | https://doi.org/10.1074/jbc.M101807200 |
ISSN: | 0021-9258 |
Appears in Collections: | Articles publicats en revistes (Patologia i Terapèutica Experimental) Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia) Articles publicats en revistes (Bioquímica i Biomedicina Molecular) |
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