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http://hdl.handle.net/2445/178888
Title: | Parallel Clamps and Polypurine Hairpins (PPRH) for Gene Silencing and Triplex-Affinity Capture: Design, Synthesis, and Use |
Author: | Aviñó Andrés, Anna Eritja i Casadellà, Ramon Ciudad i Gómez, Carlos Julián Noé Mata, Verónica |
Keywords: | ADN Teràpia genètica Reparació de l'ADN DNA Gene therapy DNA repair |
Issue Date: | 26-Mar-2019 |
Publisher: | John Wiley & Sons |
Abstract: | Nucleic acid triplexes are formed when a DNA or RNA oligonucleotide binds to a polypurine-polypyrimidine-rich sequence. Triplexes have wide therapeutic applications such as gene silencing or site-specific mutagenesis. In addition, protocols based on triplex-affinity capture have been used for detecting nucleic acids in biosensing platforms. In this article, the design, synthesis, and use of parallel clamps and polypurine-reversed hairpins (PPRH) to bind to target polypyrimidine targets are described. The combination of the polypurine Watson-Crick strand with the triplex-forming strand in a single molecule produces highly stable triplexes allowing targeting of single- and double-stranded nucleic acid sequences. On the other hand, PPRHs are easily prepared and work at nanomolar range, like siRNAs, and at a lower concentration than that needed for antisense ODNs or TFOs. However, the stability of PPRHs is higher than that of siRNAs. In addition, PPRHs circumvent off-target effects and are non-immunogenic. |
Note: | Versió postprint del document publicat a: https://doi.org/10.1002/cpnc.78 |
It is part of: | Current protocols in nucleic acid chemistry, 2019, vol. 77, num. 1, p. e78 |
URI: | http://hdl.handle.net/2445/178888 |
Related resource: | https://doi.org/10.1002/cpnc.78 |
ISSN: | 1934-9289 |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Fisiologia) |
Files in This Item:
File | Description | Size | Format | |
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690751.pdf | 1.07 MB | Adobe PDF | View/Open |
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