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http://hdl.handle.net/2445/178888
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DC Field | Value | Language |
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dc.contributor.author | Aviñó Andrés, Anna | - |
dc.contributor.author | Eritja i Casadellà, Ramon | - |
dc.contributor.author | Ciudad i Gómez, Carlos Julián | - |
dc.contributor.author | Noé Mata, Verónica | - |
dc.date.accessioned | 2021-07-08T10:32:28Z | - |
dc.date.available | 2021-07-08T10:32:28Z | - |
dc.date.issued | 2019-03-26 | - |
dc.identifier.issn | 1934-9289 | - |
dc.identifier.uri | http://hdl.handle.net/2445/178888 | - |
dc.description.abstract | Nucleic acid triplexes are formed when a DNA or RNA oligonucleotide binds to a polypurine-polypyrimidine-rich sequence. Triplexes have wide therapeutic applications such as gene silencing or site-specific mutagenesis. In addition, protocols based on triplex-affinity capture have been used for detecting nucleic acids in biosensing platforms. In this article, the design, synthesis, and use of parallel clamps and polypurine-reversed hairpins (PPRH) to bind to target polypyrimidine targets are described. The combination of the polypurine Watson-Crick strand with the triplex-forming strand in a single molecule produces highly stable triplexes allowing targeting of single- and double-stranded nucleic acid sequences. On the other hand, PPRHs are easily prepared and work at nanomolar range, like siRNAs, and at a lower concentration than that needed for antisense ODNs or TFOs. However, the stability of PPRHs is higher than that of siRNAs. In addition, PPRHs circumvent off-target effects and are non-immunogenic. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | John Wiley & Sons | - |
dc.relation.isformatof | Versió postprint del document publicat a: https://doi.org/10.1002/cpnc.78 | - |
dc.relation.ispartof | Current protocols in nucleic acid chemistry, 2019, vol. 77, num. 1, p. e78 | - |
dc.relation.uri | https://doi.org/10.1002/cpnc.78 | - |
dc.rights | (c) John Wiley & Sons, 2019 | - |
dc.source | Articles publicats en revistes (Bioquímica i Fisiologia) | - |
dc.subject.classification | ADN | - |
dc.subject.classification | Teràpia genètica | - |
dc.subject.classification | Reparació de l'ADN | - |
dc.subject.other | DNA | - |
dc.subject.other | Gene therapy | - |
dc.subject.other | DNA repair | - |
dc.title | Parallel Clamps and Polypurine Hairpins (PPRH) for Gene Silencing and Triplex-Affinity Capture: Design, Synthesis, and Use | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 690751 | - |
dc.date.updated | 2021-07-08T10:32:28Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Fisiologia) |
Files in This Item:
File | Description | Size | Format | |
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690751.pdf | 1.07 MB | Adobe PDF | View/Open |
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