Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/179355
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chavarria-Miró, Gemma | - |
dc.contributor.author | Castellarnau Serra, Montserrat de | - |
dc.contributor.author | Fuentes, Cristina | - |
dc.contributor.author | D'Andrea Rodríguez-Vida, Lucía | - |
dc.contributor.author | Pérez-Rodríguez, Francisco Javier | - |
dc.contributor.author | Beguiristain, Nerea | - |
dc.contributor.author | Bosch, Albert | - |
dc.contributor.author | Guix Arnau, Susana | - |
dc.contributor.author | Pintó Solé, Rosa María | - |
dc.date.accessioned | 2021-07-22T12:03:07Z | - |
dc.date.available | 2021-07-22T12:03:07Z | - |
dc.date.issued | 2021-02-18 | - |
dc.identifier.issn | 1664-302X | - |
dc.identifier.uri | http://hdl.handle.net/2445/179355 | - |
dc.description.abstract | The available cell-adapted hepatitis A virus (HAV) strains show a very slow replication phenotype hampering the affordable production of antigen. A fast-growing strain characterized by the occurrence of mutations in the internal ribosome entry site (IRES), combined with changes in the codon composition has been selected in our laboratory. A characterization of the IRES activity of this fast-growing strain (HM175-HP; HP) vs. its parental strain (HM175; L0) was assessed in two cell substrates used in vaccine production (MRC-5 and Vero cells) compared with the FRhK-4 cell line in which its selection was performed. The HP-derived IRES was significantly more active than the L0-derived IRES in all cells tested and both IRES were more active in the FRhK-4 cells. The translation efficiency of the HP-derived IRES was also much higher than the L0-derived IRES, particularly, in genes with a HP codon usage background. These results correlated with a higher virus production in a shorter time for the HP strain compared to the L0 strain in any of the three cell lines tested, and of both strains in the FRhK-4 cells compared to Vero and MRC-5 cells. The addition of wortmannin resulted in the increase of infectious viruses and antigen in the supernatant of FRhK-4 infected cells, independently of the strain. Finally, the replication of both strains in a clone of FRhK-4 cells adapted to grow with synthetic sera was optimal and again the HP strain showed higher yields. | - |
dc.format.extent | 10 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Frontiers Media | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.3389/fmicb.2021.642267 | - |
dc.relation.ispartof | Frontiers in Microbiology, 2021, vol. 12, num. 255 | - |
dc.relation.uri | https://doi.org/10.3389/fmicb.2021.642267 | - |
dc.rights | cc-by (c) Chavarria-Miró, Gemma et al., 2021 | - |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | - |
dc.source | Articles publicats en revistes (Genètica, Microbiologia i Estadística) | - |
dc.subject.classification | Virus de l'hepatitis A | - |
dc.subject.classification | Vacunació | - |
dc.subject.other | Hepatitis A virus | - |
dc.subject.other | Vaccination | - |
dc.title | Advances for the Hepatitis A virus antigen production using a virus strain with codon frequency optimization adjustments in specific locations | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 711677 | - |
dc.date.updated | 2021-07-22T12:03:07Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 33679679 | - |
Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
711677.pdf | 5.24 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License