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Title: | Performance of an affordable urine self-sampling method for human papillomavirus detection in Mexican women |
Author: | Hernández López, Rubí Hermosillo, Luis León Maldonado, Leith Velázquez Cruz, Rafael Torres Ibarra, Leticia Lazcano Ponce, Eduardo Lorincz, Attila Tibor Wheeler, Cosette M. Bosch José, Francesc Xavier, 1947- Cuzick, Jack Rivera Paredez, Berenice Nedjai, Belinda Salmerón, Jorge |
Keywords: | Papil·lomavirus Anàlisi d'orina Papillomaviruses Urine--Analysis |
Issue Date: | 21-Jul-2021 |
Publisher: | Public Library of Science (PLoS) |
Abstract: | Introduction: Urine self-sampling for human papillomavirus (HPV)-based cervical cancer screening is a non-invasive method that offers several logistical advantages and high acceptability, reducing barriers related to low screening coverage. This study developed and evaluated the performance of a low-cost urine self-sampling method for HPV-testing and explored the acceptability and feasibility of potential implementation of this alternative in routine screening. Methods: A series of sequential laboratory assays examined the impact of several pre-analytical conditions for obtaining DNA from urine and subsequent HPV detection. Initially, we assessed the effect of ethylaminediaminetetraacetic acid (EDTA) as a DNA preservative examining several variables including EDTA concentration, specimen storage temperature, time between urine collection and DNA extraction, and first-morning micturition versus convenience sample collection. We further evaluated the agreement of HPV-testing between urine and clinician-collected cervical samples among 95 women. Finally, we explored the costs of self-sampling supplies as well as the acceptability and feasibility of urine self-sampling among women and healthcare workers. Results: Our results revealed higher DNA concentrations were obtained when using a 40mM EDTA solution, storing specimens at 25°C and extracting DNA within 72 hrs. of urine collection, regardless of using first-morning micturition or a convenience sampling. We observed good agreement (Kappa = 0.72) between urine and clinician-collected cervical samples for HPV detection. Furthermore, urine self-sampling was an affordable method (USD 1.10), well accepted among cervical cancer screening users, healthcare workers, and decision-makers. Conclusion: These results suggest urine self-sampling is feasible and appropriate alternative for HPV-testing in HPV-based screening programs in lower-resource contexts. |
Note: | Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0254946 |
It is part of: | PLOS ONE, 2021, vol. 16, num. 7 |
URI: | http://hdl.handle.net/2445/179962 |
Related resource: | https://doi.org/10.1371/journal.pone.0254946 |
ISSN: | 1932-6203 |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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journal.pone.0254946.pdf | 1.02 MB | Adobe PDF | View/Open |
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