Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/182016
Title: | Mechanical compartmentalization of the intestinal organoid enables crypt folding and collective cell migration |
Author: | Pérez González, Carlos Ceada, Gerardo Greco, Francesco Matejčić, Marija Batlle, Eduard Gómez González, Manuel Castro, Natalia Menendez, Anghara Kale, Sohan Krndija, Denis Clark, Andrew G. Gannavarapu, Venkata Ram Álvarez Varela, Adrián Roca Cusachs, Pere Vignjevic, Danijela Matic Arroyo, Marino Trepat Guixer, Xavier |
Keywords: | Intestins Migració cel·lular Intestines Cell migration |
Issue Date: | 21-Jun-2021 |
Publisher: | Nature Publishing Group |
Abstract: | Intestinal organoids capture essential features of the intestinal epithelium such as crypt folding, cellular compartmentalization and collective movements. Each of these processes and their coordination require patterned forces that are at present unknown. Here we map three-dimensional cellular forces in mouse intestinal organoids grown on soft hydrogels. We show that these organoids exhibit a non-monotonic stress distribution that defines mechanical and functional compartments. The stem cell compartment pushes the extracellular matrix and folds through apical constriction, whereas the transit amplifying zone pulls the extracellular matrix and elongates through basal constriction. The size of the stem cell compartment depends on the extracellular-matrix stiffness and endogenous cellular forces. Computational modelling reveals that crypt shape and force distribution rely on cell surface tensions following cortical actomyosin density. Finally, cells are pulled out of the crypt along a gradient of increasing tension. Our study unveils how patterned forces enable compartmentalization, folding and collective migration in the intestinal epithelium. |
Note: | Versió postprint del document publicat a: https://doi.org/10.1038/s41556-021-00699-6 |
It is part of: | Nature Cell Biology, 2021, vol. 23, num. 7, p. 745-757 |
URI: | http://hdl.handle.net/2445/182016 |
Related resource: | https://doi.org/10.1038/s41556-021-00699-6 |
ISSN: | 1465-7392 |
Appears in Collections: | Articles publicats en revistes (Biomedicina) Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC)) Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona)) Publicacions de projectes de recerca finançats per la UE |
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