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http://hdl.handle.net/2445/183115
Title: | Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification |
Author: | Rodriguez Temporal, David Alcaide Fernández de Vega, Fernando Mareković, Ivana O’Connor, James Anthony Gorton, Rebecca Ingen, Jakko van Bossche, An van den Héry-Arnaud, Genevieve Beauruelle, Clémence Orth-Höller, Dorothea Palacios Gutiérrez, Juan José Tudó i Vilanova, Griselda Bou, Germán Ceyssens, Pieter-Jan Garrigó, Montserrat González Martín, Julián Greub, Gilbert Hrabak, Jaroslav Ingebretsen, André Mediavilla Gradolph, Maria Concepción Oviaño, Marina Palop, Begoña Pranada, Arthur B. Quiroga, Lidia Ruiz Serrano, Maria Jesús Rodríguez Sánchez, Belén |
Keywords: | Malalties del pulmó Bacteris patògens Pulmonary diseases Pathogenic bacteria |
Issue Date: | 24-Jan-2022 |
Publisher: | Springer Science and Business Media LLC |
Abstract: | The ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on the reproducibility of MALDI-TOF have been performed so far, none on mycobacteria. The aim of this study was to evaluate the reproducibility of MALDI-TOF for the identification of NTM in 15 laboratories in 9 European countries. A total of 98 NTM clinical isolates were grown on Lowenstein-Jensen. Biomass was collected in tubes with water and ethanol, anonymized and sent out to the 15 participating laboratories. Isolates were identified using MALDI Biotyper (Bruker Daltonics). Up to 1330 MALDI-TOF identifications were collected in the study. A score >= 1.6 was obtained for 100% of isolates in 5 laboratories (68.2-98.6% in the other). Species-level identification provided by MALDI-TOF was 100% correct in 8 centres and 100% correct to complex-level in 12 laboratories. In most cases, the misidentifications obtained were associated with closely related species. The variability observed for a few isolates could be due to variations in the protein extraction procedure or to MALDI-TOF system status in each centre. In conclusion, MALDI-TOF showed to be a highly reproducible method and suitable for its implementation for NTM identification. |
Note: | Reproducció del document publicat a: https://doi.org/10.1038/s41598-022-05315-7 |
It is part of: | Scientific Reports, 2022, vol 12, num 1 |
URI: | http://hdl.handle.net/2445/183115 |
Related resource: | https://doi.org/10.1038/s41598-022-05315-7 |
ISSN: | 2045-2322 |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) Articles publicats en revistes (Patologia i Terapèutica Experimental) Articles publicats en revistes (ISGlobal) Articles publicats en revistes (Fonaments Clínics) |
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