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Title: Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification
Author: Rodriguez Temporal, David
Alcaide Fernández de Vega, Fernando
Mareković, Ivana
O’Connor, James Anthony
Gorton, Rebecca
Ingen, Jakko van
Bossche, An van den
Héry-Arnaud, Genevieve
Beauruelle, Clémence
Orth-Höller, Dorothea
Palacios Gutiérrez, Juan José
Tudó i Vilanova, Griselda
Bou, Germán
Ceyssens, Pieter-Jan
Garrigó, Montserrat
González Martín, Julián
Greub, Gilbert
Hrabak, Jaroslav
Ingebretsen, André
Mediavilla Gradolph, Maria Concepción
Oviaño, Marina
Palop, Begoña
Pranada, Arthur B.
Quiroga, Lidia
Ruiz Serrano, Maria Jesús
Rodríguez Sánchez, Belén
Keywords: Malalties del pulmó
Bacteris patògens
Pulmonary diseases
Pathogenic bacteria
Issue Date: 24-Jan-2022
Publisher: Springer Science and Business Media LLC
Abstract: The ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on the reproducibility of MALDI-TOF have been performed so far, none on mycobacteria. The aim of this study was to evaluate the reproducibility of MALDI-TOF for the identification of NTM in 15 laboratories in 9 European countries. A total of 98 NTM clinical isolates were grown on Lowenstein-Jensen. Biomass was collected in tubes with water and ethanol, anonymized and sent out to the 15 participating laboratories. Isolates were identified using MALDI Biotyper (Bruker Daltonics). Up to 1330 MALDI-TOF identifications were collected in the study. A score >= 1.6 was obtained for 100% of isolates in 5 laboratories (68.2-98.6% in the other). Species-level identification provided by MALDI-TOF was 100% correct in 8 centres and 100% correct to complex-level in 12 laboratories. In most cases, the misidentifications obtained were associated with closely related species. The variability observed for a few isolates could be due to variations in the protein extraction procedure or to MALDI-TOF system status in each centre. In conclusion, MALDI-TOF showed to be a highly reproducible method and suitable for its implementation for NTM identification.
Note: Reproducció del document publicat a:
It is part of: Scientific Reports, 2022, vol 12, num 1
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ISSN: 2045-2322
Appears in Collections:Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))
Articles publicats en revistes (Patologia i Terapèutica Experimental)
Articles publicats en revistes (ISGlobal)
Articles publicats en revistes (Fonaments Clínics)

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