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Title: A proteomics approach to decipher the molecular nature of planarian stem cells.
Author: Fernandez-Taboada, Enrique
Rodríguez Esteban, Gustavo
Saló i Boix, Emili
Abril Ferrando, Josep Francesc, 1970-
Keywords: Proteòmica
Regeneració (Biologia)
Cèl·lules mare
Regeneration (Biology)
Stem cells
Issue Date: 28-Feb-2011
Publisher: BioMed Central
Abstract: Background In recent years, planaria have emerged as an important model system for research into stem cells and regeneration. Attention is focused on their unique stem cells, the neoblasts, which can differentiate into any cell type present in the adult organism. Sequencing of the Schmidtea mediterranea genome and some expressed sequence tag projects have generated extensive data on the genetic profile of these cells. However, little information is available on their protein dynamics. Results We developed a proteomic strategy to identify neoblast-specific proteins. Here we describe the method and discuss the results in comparison to the genomic high-throughput analyses carried out in planaria and to proteomic studies using other stem cell systems. We also show functional data for some of the candidate genes selected in our proteomic approach. Conclusions We have developed an accurate and reliable mass-spectra-based proteomics approach to complement previous genomic studies and to further achieve a more accurate understanding and description of the molecular and cellular processes related to the neoblasts.
Note: Reproducció del document publicat a:
It is part of: BMC Genomics, 2011, 12:133
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ISSN: 1471-2164
Appears in Collections:Articles publicats en revistes (Genètica, Microbiologia i Estadística)

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